| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
以下是关于NUDC抗体的3篇文献示例(注：部分内容为模拟生成，仅供参考)：
1. **文献名称**：**"NUDC is essential for mitotic progression and chromosome segregation in mammalian cells"**
**作者**：Aumais JP, et al.
**摘要**：本研究通过特异性NUDC抗体进行免疫荧光染色，发现NUDC蛋白在有丝分裂期间定位在纺锤体区域，并证明其通过调控微管动力学保障染色体的正确分离，敲低NUDC导致细胞分裂异常。
2. **文献名称**：**"Overexpression of NUDC correlates with poor prognosis in colorectal cancer"**
**作者**：Li Y, et al.
**摘要**：利用NUDC多克隆抗体对结直肠癌组织进行免疫组化分析，发现NUDC在肿瘤组织中高表达，且与患者生存率负相关，提示其可能作为癌症预后标志物及治疗靶点。
3. **文献名称**：**"Development of a high-affinity monoclonal antibody against NUDC for neurodegenerative disease research"**
**作者**：Smith KA, et al.
**摘要**：研究团队开发了一种新型NUDC单克隆抗体，通过Western blot和免疫共沉淀验证其高特异性，并应用于阿尔茨海默病模型，发现NUDC表达异常与神经元轴突运输缺陷相关。
4. **文献名称**：**"NUDC antibody-based drug screening identifies potential inhibitors for leukemia therapy"**
**作者**：Zhang R, et al.
**摘要**：采用NUDC抗体建立高通量筛选平台，筛选靶向NUDC的小分子化合物，发现部分化合物可通过抑制NUDC-动力蛋白相互作用，显著降低白血病细胞的增殖能力。
(注：以上文献信息为示例性质，实际研究中请通过PubMed或Web of Science等数据库检索具体文献。)
NUDC (Nuclear Distribution Protein C) is a conserved eukaryotic protein involved in mitotic spindle organization, chromosome segregation, and nuclear migration during cell division. It interacts with the dynein-dynactin motor complex and microtubules, facilitating proper microtubule dynamics and mitotic progression. Dysregulation of NUDC is linked to chromosomal instability, developmental defects, and cancer progression.
NUDC antibodies are immunological tools developed to detect and study this protein's expression, localization, and function. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate NUDC's role in cell cycle regulation, particularly in cancer research where its overexpression correlates with tumor aggressiveness and poor prognosis. Some studies also explore NUDC's involvement in neurological disorders due to its neuronal migration functions.
Commercial NUDC antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides as antigens. Validation includes testing in knockout cell lines or tissues to confirm specificity. Challenges remain in distinguishing isoforms or post-translationally modified forms. Recent research leverages these antibodies to dissect NUDC's emerging roles in viral infection responses and primary cilia function, highlighting its multifaceted cellular importance.
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