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产品描述：人α-N-乙酰氨基半乳糖苷酶(NAGA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、唾液、组织匀浆样本中的NAGA含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，本产品适用于基础医学研究、代谢通路分析或疾病模型构建等领域，支持多类型样本检测，为探索NAGA在糖基化修饰、溶酶体功能调控及代谢相关疾病中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NAGA ELISA Kit; Alpha-N-acetylgalactosaminidase ELISA Kit; EC 3.2.1.49 ELISA Kit; Alpha-galactosidase B ELISA Kit
缩写：NAGA
Uniprot No.：P17050
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, saliva, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NAGA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 82-90 | |||||
| 1:8 | Average % | 105 | ||||
| Range % | 100-109 | |||||
回收率：
| The recovery of human NAGA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.2462.1522.1991.980 201.8921.8151.8541.635 101.4651.5041.4851.266 51.1271.1791.1530.934 2.50.8740.8360.8550.636 1.250.5410.5680.5550.336 0.6250.3620.3490.3560.137 00.2210.2170.219 | ||||||
货期：3-5 working days
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