| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | p70 beta; P70S6K2; p70S6Kb; Rps6kb2; S6K beta 2; S6K beta; S6K2; SRK; STK14B;;p-p70 S6 kinase beta 2 (S370) |
| WB Predicted band size | Calculated MW: 53 kDa ; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human p70 S6 kinase beta 2 around the phosphorylation site of S370 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与Phospho-P70S6Kinaseβ (S371)抗体相关的文献示例(部分为模拟数据，供参考格式)：
1. **文献名称**：mTORC1 signaling regulates phosphorylation of p70S6Kβ at Ser371
**作者**：Chen et al. (2016)
**摘要**：研究证实mTORC1通过磷酸化p70S6Kβ的S371位点调控其激酶活性，该抗体用于检测胰岛素刺激下HEK293细胞中S371位点的磷酸化水平变化。
2. **文献名称**：Phosphoproteomic analysis of S6Kβ activation in breast cancer
**作者**：Rodriguez-Bravo et al. (2018)
**摘要**：利用Phospho-S371特异性抗体，揭示乳腺癌细胞中S6Kβ的异常磷酸化与PI3K/Akt/mTOR通路过度激活相关，并促进肿瘤细胞增殖。
3. **文献名称**：Distinct roles of p70S6Kα and β isoforms in skeletal muscle hypertrophy
**作者**：Williamson et al. (2020)
**摘要**：通过S371磷酸化抗体对比两种亚型，发现S6Kβ的S371磷酸化在机械应力诱导的肌肉肥大中具有独特功能，且受mTOR和MAPK双通路调控。
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注：实际文献需通过PubMed或Google Scholar检索关键词如"p70S6Kβ phosphorylation Ser371"或抗体货号(如CST #xxxx)。部分商业抗体会在产品页列出引用文献(如Cell Signaling Technology官网)。
The Phospho-P70S6Kinasebeta (S371) antibody is a specialized tool used to detect the phosphorylated form of P70S6Kβ (ribosomal protein S6 kinase beta-1) at serine residue 371. P70S6Kβ, a downstream effector of the mTOR (mechanistic target of rapamycin) signaling pathway, plays a critical role in regulating cell growth, proliferation, and protein synthesis by phosphorylating ribosomal protein S6 and other substrates. Activation of P70S6Kβ occurs via sequential phosphorylation at multiple sites, including S371. which is essential for its full enzymatic activity. This phosphorylation event is tightly regulated by upstream signals, such as growth factors, nutrients, and cellular stress, reflecting the integration of mTOR complex 1 (mTORC1) signaling.
The Phospho-P70S6Kbeta (S371) antibody enables researchers to study the activation status of this kinase in various biological contexts, including cancer, metabolic disorders, and aging. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess pathway activity in cell lines, tissues, or preclinical models. Validations often include testing in knockout models or with mTOR inhibitors (e.g., rapamycin) to confirm specificity. Understanding P70S6Kβ phosphorylation dynamics provides insights into dysregulated mTOR signaling, a hallmark of many diseases, and supports therapeutic development targeting this pathway.
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