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产品描述：人E3泛素蛋白连接酶MYLIP(MYLIP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MYLIP含量。MYLIP 是一个关键靶点。它广泛参与细胞内脂质代谢调控等过程。研究表明，MYLIP 可影响相关脂蛋白的表达与代谢，其机制涉及与特定蛋白相互作用等。对它的深入研究有助于理解脂质代谢紊乱疾病，也为开发针对性治疗手段提供方向。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本产品适用于基础科研领域中对MYLIP蛋白表达量的动态监测，例如探究药物干预、基因编辑或疾病模型中MYLIP的功能变化，为脂代谢信号通路研究、动脉硬化机制分析及神经退行性疾病相关实验提供可靠的定量工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BM-023 ELISA Kit; BZF1 ELISA Kit; E3 ubiquitin protein ligase MYLIP ELISA Kit; E3 ubiquitin-protein ligase MYLIP ELISA Kit; Idol ELISA Kit; Inducible degrader of the LDL-receptor ELISA Kit; Lipoprotein receptor ELISA Kit; MIR ELISA Kit; MYLIP ELISA Kit; MYLIP_HUMAN ELISA Kit; Myosin regulatory light chain interacting protein ELISA Kit; PP5242 ELISA Kit
缩写：MYLIP
Uniprot No.：Q8WY64
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MYLIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 105 | |||||
| Range % | 100-110 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 92-99 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 84-94 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 90-96 | ||||||
回收率：
| The recovery of human MYLIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 99-103 | |||||
| EDTA plasma (n=4) | 90 | 85-95 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 10002.7782.8642.8212.689 5002.2702.3052.2882.156 2501.5201.5321.5261.394 1250.9190.9560.9380.806 62.50.5540.5240.5390.407 31.20.4080.3980.4030.271 15.60.2630.2580.2610.129 00.1290.1340.132 | |||||||
货期：3-5 working days
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