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产品描述：人肌球蛋白-11(MYH11)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MYH11含量。MYH11是重要的靶点。它是一种肌球蛋白重链，参与平滑肌收缩等生理过程。其异常表达或功能改变与多种疾病相关，如血管疾病等。研究机制多聚焦于其在疾病发生发展中的信号通路作用，以及与其他分子的相互作用，以探索潜在治疗策略。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于科研领域中对平滑肌相关疾病机制、肿瘤微环境调控或血管功能研究中的MYH11蛋白表达水平检测，为体外实验提供稳定可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AAT4 ELISA Kit; DKFZp686D10126 ELISA Kit; DKFZp686D19237 ELISA Kit; FAA4 ELISA Kit; FLJ35232 ELISA Kit; MGC126726 ELISA Kit; MGC32963 ELISA Kit; MYH 11 ELISA Kit; MYH11 ELISA Kit; MYH11_HUMAN ELISA Kit; Myosin 11 ELISA Kit; Myosin heavy chain 11 ELISA Kit; Myosin heavy chain 11 smooth muscle ELISA Kit; Myosin heavy chain ELISA Kit; Myosin heavy chain smooth muscle isoform ELISA Kit; Myosin heavy polypeptide 11 smooth muscle ELISA Kit; Myosin-11 ELISA Kit; SMHC ELISA Kit; SMMHC ELISA Kit; smooth muscle isoform ELISA Kit; Smooth muscle myosin heavy chain 11 isoform SM2 ELISA Kit; Smooth muscle myosin heavy chain isoform SM2 ELISA Kit
缩写：MYH11
Uniprot No.：P35749
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MYH11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-105 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-101 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human MYH11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 94-101 | ||||
| EDTA plasma (n=4) | 88 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.2952.4982.3972.2588001.5431.4421.4931.3544000.8910.8430.8670.7282000.5280.5370.5330.3941000.3220.3350.3290.190500.2550.2670.2610.122250.1740.1790.1770.03800.1380.1390.139 | ||||||
货期：3-5 working days
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