首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人髓样分化因子88(MyD88)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MYD88含量。MYD88是一种重要的靶点。它是髓样分化因子 88，在 Toll 样受体和白细胞介素-1 受体信号通路中起关键衔接作用。研究发现其在多种疾病尤其淋巴瘤等恶性肿瘤发生发展中异常激活，通过调节下游信号分子参与炎症反应和肿瘤细胞存活、增殖等，是潜在治疗靶点。试剂盒检测范围为0.312ng/mL-20ng/mL，适用于科研领域中对MyD88表达动态的研究，例如探索病原体感染后免疫信号激活机制、炎症相关疾病模型中TLR通路的分子调控网络，或肿瘤微环境中免疫细胞的功能状态分析。为免疫学、肿瘤学及感染性疾病的基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mutant myeloid differentiation primary response 88 ELISA Kit; MYD 88 ELISA Kit; Myd88 ELISA Kit; MYD88_HUMAN ELISA Kit; MYD88D ELISA Kit; Myeloid differentiation marker 88 ELISA Kit; Myeloid differentiation primary response 88 ELISA Kit; Myeloid differentiation primary response gene (88) ELISA Kit; Myeloid differentiation primary response gene 88 ELISA Kit; Myeloid differentiation primary response gene ELISA Kit; Myeloid differentiation primary response protein MyD88 ELISA Kit; OTTHUMP00000161718 ELISA Kit; OTTHUMP00000208595 ELISA Kit; OTTHUMP00000209058 ELISA Kit; OTTHUMP00000209059 ELISA Kit; OTTHUMP00000209060 ELISA Kit
缩写：MYD88
Uniprot No.：Q99836
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312ng/mL-20ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MyD88 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 97-104 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 96-102 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human MyD88 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-90 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.4882.5202.5042.331 102.0452.0682.0571.884 51.3811.3521.3671.194 2.50.7460.7880.7670.594 1.250.4650.4680.4670.294 0.6250.3520.3680.3600.187 0.3120.2670.2510.2590.086 00.1720.1740.173 | ||||||
货期：3-5 working days
×
