| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CCT alpha; CCT; CCT1; Ccta; p63; TCP 1 alpha; TCP1 Tp63; TRic;;TCP1 alpha |
| WB Predicted band size | 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human TCP1 alpha |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于TCP1alpha抗体的3篇参考文献示例(虚构，仅供格式参考)：
1. **文献名称**：*"Characterization of TCP1alpha antibody for detecting chaperonin complex in mammalian cells"*
**作者**：Smith J et al.
**摘要**：本研究开发了一种特异性识别TCP1alpha(TRiC复合体亚基)的单克隆抗体，验证了其在免疫印迹(WB)和免疫荧光(IF)中的应用，揭示了其在细胞应激条件下与错误折叠蛋白的共定位。
2. **文献名称**：*"TCP1alpha expression correlates with tumor progression in glioblastoma"*
**作者**：Chen L et al.
**摘要**：通过抗TCP1alpha抗体的免疫组化分析，发现TCP1alpha在胶质母细胞瘤中高表达，且与患者预后不良相关，提示其作为癌症生物标志物的潜力。
3. **文献名称**：*"A novel polyclonal antibody reveals TCP1alpha interaction with amyloid-beta in Alzheimer's disease models"*
**作者**：Wang Y et al.
**摘要**：利用新型多克隆抗体，证实了TCP1alpha与阿尔茨海默病模型中β-淀粉样蛋白的相互作用，为研究蛋白质错误折叠机制提供了工具。
(注：以上文献为示例，实际研究中请通过PubMed或学术数据库查询真实文献。)
The TCP1alpha antibody targets the TCP1 (T-complex protein 1) alpha subunit, a critical component of the chaperonin-containing TCP-1 (CCT) complex. This evolutionarily conserved complex, also known as the TRiC (TCP-1 Ring Complex), plays an essential role in ATP-dependent protein folding, particularly for cytoskeletal proteins like actin and tubulin. The CCT complex consists of eight to nine distinct subunits arranged in a double-ring structure, with TCP1alpha (CCT1) being one of the principal subunits involved in substrate recognition and binding.
Research on TCP1alpha has gained attention due to its multifaceted roles in cellular processes. Beyond protein folding, it participates in cell cycle regulation, stress responses, and maintaining proteostasis. Dysregulation of TCP1alpha expression has been linked to various pathologies, including cancer progression, neurodegenerative disorders, and viral infections. For instance, elevated TCP1alpha levels are observed in multiple cancer types, where it may support tumor cell proliferation by stabilizing oncoproteins.
The TCP1alpha antibody is widely utilized in molecular and cellular biology to investigate protein folding mechanisms, monitor CCT complex assembly, and explore disease-associated molecular pathways. It enables detection of TCP1alpha expression through techniques like Western blotting, immunofluorescence, and immunoprecipitation. Recent studies also employ this antibody to dissect its potential as a therapeutic target, particularly in cancers dependent on chaperonin-mediated proteostasis. Its specificity and reliability make it a valuable tool for both basic research and clinical applications.
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