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产品描述：人粘蛋白5AC(MUC5AC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液样本中的MUC5AC含量。MUC5AC是重要的黏蛋白基因，其编码的黏蛋白在呼吸道等部位黏膜表面起保护屏障作用。但过度表达会堵塞气道，与哮喘、慢阻肺等呼吸道疾病发生发展密切相关。研究多聚焦于抑制其表达及调控机制，以探索治疗相关疾病的新靶点。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，科研人员可通过该产品探究呼吸道炎症模型中黏液分泌调控机制、评估肿瘤微环境中黏蛋白的表达特征，或结合尿液样本分析泌尿系统相关疾病中 MUC5AC 的潜在生物学意义。为分子机制探索及生物标志物筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MUC5AC ELISA Kit; MUC5 ELISA Kit; Mucin-5AC ELISA Kit; MUC-5AC ELISA Kit; Gastric mucin ELISA Kit; Major airway glycoprotein ELISA Kit; Mucin-5 subtype AC ELISA Kit; tracheobronchial ELISA Kit; Tracheobronchial mucin ELISA Kit; TBM ELISA Kit
缩写：MUC5AC
Uniprot No.：P98088
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, urine
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MUC5AC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 87-99 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 87-96 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 94-106 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-97 | |||||
回收率：
| The recovery of human MUC5AC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 95-93 | ||||
| EDTA plasma (n=4) | 94 | 91-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected10002.4522.5632.5082.3755001.9491.9641.9571.8242501.3581.4311.3951.2621250.8120.8320.8220.68962.50.5360.5760.5560.42331.20.3420.3560.3490.21615.60.2410.2490.2450.11200.1320.1340.133 | ||||||
本试剂盒所含材料：
A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human MUC5AC antibody.This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labled MUC5AC antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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