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产品描述：人粘蛋白-4(MUC4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MUC4含量。MUC4是一种跨膜黏蛋白，在多种癌症中异常表达，与肿瘤的转移和抗凋亡相关。其研究机制涉及通过影响细胞黏附参与血栓形成，以及通过HER2介导的胃癌耐药机制。MUC4突变在经典型阵发性睡眠性血红蛋白尿症患者中与血栓事件相关，可作为新的评估血栓形成风险的预测因素。试剂盒检测范围为0.625 ng/mL-40 ng/mL，研究者可将其应用于肿瘤微环境研究、上皮屏障功能评估或疾病相关生物标志物筛查等方向，例如探索MUC4在消化道肿瘤、呼吸系统疾病或组织修复过程中的作用机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ascites sialoglycoprotein 1 ELISA Kit; Ascites sialoglycoprotein 2 ELISA Kit; Ascites sialoglycoprotein ELISA Kit; ASGP ELISA Kit; ASGP-1 ELISA Kit; ASGP-2 ELISA Kit; HSA276359 ELISA Kit; MUC 4 ELISA Kit; MUC-4 ELISA Kit; Muc4 ELISA Kit; MUC4_HUMAN ELISA Kit; Mucin 4 ELISA Kit; Mucin 4 cell surface associated ELISA Kit; Mucin 4 tracheobronchial ELISA Kit; Mucin-4 beta chain ELISA Kit; Pancreatic adenocarcinoma mucin ELISA Kit; Testis mucin ELISA Kit; Tracheobronchial mucin ELISA Kit; Tracheobronchial mucin Fragment ELISA Kit
缩写：MUC4
Uniprot No.：Q99102
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MUC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 89-96 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 98-107 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-97 | |||||
回收率：
| The recovery of human MUC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-98 | ||||
| EDTA plasma (n=4) | 88 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.2352.3542.2952.163201.8161.7051.7611.629101.2241.2471.2361.10450.8010.8230.8120.6802.50.4530.4640.4590.3271.250.3120.3240.3180.1860.6250.2240.2280.2260.09400.1310.1320.132 | ||||||
货期：3-5 working days
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