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Rabbit Monoclonal AP2S1 Antibody

  • 中文名: AP2S1抗体
  • 别    名: AP17; AP17 delta; Ap2s1; CLAPS2; Sigma2 adaptin;;AP2S1
货号: IPDX18705
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20-1/50 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/20-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesAP17; AP17 delta; Ap2s1; CLAPS2; Sigma2 adaptin;;AP2S1
WB Predicted band size17 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human AP2S1
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是3篇与AP2S1抗体相关的模拟参考文献示例(注:具体内容为基于领域知识的模拟,建议通过学术数据库核实真实文献):

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1. **文献名称**: "AP2S1 regulates calcium-sensing receptor signaling through clathrin-mediated endocytosis"

**作者**: Smith A, et al.

**摘要**: 本研究揭示了AP2S1作为衔接蛋白复合体AP-2的关键亚基,在钙离子传感受体(CaSR)的内吞和信号传导中的调控作用。通过AP2S1抗体进行免疫印迹和免疫荧光实验,发现AP2S1缺失会抑制CaSR内化,导致下游MAPK信号通路异常,提示其在钙稳态中的关键功能。

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2. **文献名称**: "A recurrent heterozygous mutation in AP2S1 causes familial hypocalciuric hypercalcemia type 3"

**作者**: Li Y, et al.

**摘要**: 该研究在家族性低尿钙高钙血症(FHH)患者中发现AP2S1基因的复发性杂合突变(Arg15Leu)。利用AP2S1特异性抗体进行蛋白质稳定性分析,发现突变体导致AP-2复合物结构异常,损害细胞对胞外钙离子浓度的响应能力,为FHH的分子机制提供了新证据。

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3. **文献名称**: "AP2S1 modulates amyloid precursor protein trafficking and processing in Alzheimer’s disease models"

**作者**: Chen L, et al.

**摘要**: 研究探讨了AP2S1在阿尔茨海默病(AD)中调控淀粉样前体蛋白(APP)运输的作用。通过AP2S1抗体阻断实验,发现下调AP2S1会减少APP内吞,降低β-淀粉样蛋白(Aβ)生成,提示靶向AP2S1可能成为AD治疗的潜在策略。

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**提示**:以上为模拟示例,实际文献需通过PubMed、Web of Science等平台以关键词“AP2S1 antibody”或“AP2S1 function”检索。真实研究多聚焦于AP2S1在膜运输、遗传性疾病(如FHH)或神经退行性疾病中的机制。

背景信息

The AP2S1 antibody targets the Adaptor Protein 2 sigma subunit (AP2S1), a critical component of the heterotetrameric AP-2 complex involved in clathrin-mediated endocytosis (CME). AP2S1. a 16 kDa protein encoded on chromosome 19q13.32. stabilizes the AP-2 complex by linking its α/β2 subunits to the μ2 subunit. It plays a regulatory role in cargo recognition, membrane binding, and clathrin coat assembly during vesicle formation. AP2S1 mutations are linked to familial hypocalciuric hypercalcemia type 3 (FHH3), a calcium-sensing disorder, highlighting its role in calcium homeostasis. Researchers use AP2S1 antibodies to study CME mechanisms, intracellular trafficking, and diseases like cancer or neurological disorders associated with endocytic dysfunction. These antibodies are employed in techniques such as Western blotting, immunofluorescence, and co-immunoprecipitation to assess AP2S1 expression, localization, and interactions. Commercial AP2S1 antibodies are typically validated for specificity across human, mouse, and rat models. Recent studies also explore AP2S1's involvement in G protein-coupled receptor (GPCR) internalization and its potential as a therapeutic target. Its conserved structure and functional significance make AP2S1 a key focus in membrane biology and pathophysiology research.

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