| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Gnb2; Gnb2l1; RACK1; Transducin beta chain 2;;GNB2 |
| WB Predicted band size | Calculated MW: 37 kDa ; Observed MW: 35 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human GNB2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于GNB2抗体的3篇示例参考文献(内容为虚构，仅作格式参考)：
1. **文献名称**：*"Characterization of a High-Specificity GNB2 Antibody for Functional Proteomics Studies"*
**作者**：Zhang L, et al.
**摘要**：本研究开发了一种高特异性的GNB2多克隆抗体，通过免疫印迹和免疫荧光验证其在多种细胞系中的特异性，并用于探究GNB2在G蛋白信号通路中的动态调控机制。
2. **文献名称**：*"GNB2 Antibody-Based Profiling Identifies Dysregulation in Neurodevelopmental Disorders"*
**作者**：Chen R, et al.
**摘要**：利用GNB2抗体对患者脑组织样本进行免疫组化分析，发现GNB2蛋白表达异常与神经突触功能缺陷相关，提示其在神经发育障碍中的潜在作用。
3. **文献名称**：*"A Novel Monoclonal Antibody Targeting GNB2 Reveals Its Oncogenic Role in Breast Cancer"*
**作者**：Wang Y, et al.
**摘要**：通过构建针对GNB2的单克隆抗体，研究者证实GNB2在乳腺癌细胞中高表达，并促进肿瘤增殖和转移，为靶向治疗提供了新思路。
(注：若需真实文献，建议在PubMed或Web of Science中以“GNB2 antibody”为关键词检索。)
The GNB2 antibody targets the beta-2 subunit of heterotrimeric G proteins, which are critical components of intracellular signaling pathways. GNB2 encodes the β2 subunit, one of five β-subunit isoforms (β1–β5) that form stable dimers with γ-subunits to regulate G protein-coupled receptor (GPCR) signaling. These β/γ complexes modulate downstream effectors such as adenylyl cyclase, phospholipase C, and ion channels, influencing cellular processes like neurotransmission, hormone secretion, and cell proliferation.
GNB2 antibodies are widely used as research tools to study the expression, localization, and function of the β2 subunit in various tissues and disease models. They are essential for techniques like Western blotting, immunohistochemistry, and immunofluorescence to visualize protein levels or track dynamic interactions in signaling cascades. Dysregulation of GNB2 has been implicated in neurodevelopmental disorders, cardiovascular diseases, and cancer, where aberrant GPCR signaling may drive pathogenesis. For example, altered GNB2 expression has been linked to tumor progression and resistance to therapies targeting signaling pathways.
Validated GNB2 antibodies typically recognize conserved epitopes across species, enabling cross-disciplinary studies in model organisms. Researchers also use these antibodies to explore post-translational modifications or mutations affecting β2-subunit stability and function. Understanding GNB2's role through antibody-based assays contributes to elucidating disease mechanisms and identifying potential therapeutic targets in GPCR-related pathologies.
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