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产品描述：人粘蛋白-15(MUC15)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MUC15含量。MUC15属于黏蛋白家族成员，在多种组织中表达。它参与细胞黏附、信号传导等过程。研究发现其异常表达与肿瘤发生、发展相关，可能通过调节肿瘤细胞增殖、侵袭和转移发挥作用，有望成为肿瘤治疗的新靶点。试剂盒检测范围为125 pg/mL-8000 pg/mL，该产品适用于科研领域对MUC15蛋白表达水平的定量分析，例如在癌症发生机制、炎症相关通路研究或组织特异性表达谱分析中评估生物样本中MUC15的动态变化，为探索其在细胞通讯、疾病生物标志物筛选等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Component II ELISA Kit; Glycoprotein 4 ELISA Kit; Glycoprotein C ELISA Kit; MUC 15 ELISA Kit; MUC-15 ELISA Kit; Muc15 ELISA Kit; MUC15_HUMAN ELISA Kit; Mucin 15 ELISA Kit; Mucin 15 cell surface associated ELISA Kit; Mucin-15 ELISA Kit; PAS3 ELISA Kit; PASIII ELISA Kit
缩写：MUC15
Uniprot No.：Q8N387
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MUC15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 89-103 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 90-99 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 87-102 | ||||||
| 1:8 | Average % | 103 | |||||
| Range % | 97-108 | ||||||
回收率：
| The recovery of human MUC15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 86 | 81-90 | |||||
| EDTA plasma (n=4) | 92 | 88-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected80001.9722.0272.0001.86340001.4561.4361.4461.30920000.9020.9250.9140.77710000.5760.5830.5800.4435000.4300.4210.4260.2892500.3170.3090.3130.1761250.2500.2420.2460.10900.1400.1340.137 | |||||||
货期：3-5 working days
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