| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 4EBP1; 4EBP2; 4EBP3; eIF4E binding protein 1; eIF4E binding protein 2; eIF4E binding protein 3; PHAS1;;p-4E BP1/2/3 (T46/T46/T32) |
| WB Predicted band size | Calculated MW: 11,13 kDa ; Observed MW: 13-19 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human 4E BP1 around the phosphorylation site of T46 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Phospho-eIF4EBP1/2/3(T46+T46+T32)抗体的3篇参考文献，涵盖其在不同研究背景中的应用和机制分析：
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1. **文献名称**: **"Regulation of 4E-BP1 phosphorylation by mTOR"**
**作者**: Gingras, A.C. et al. (1999)
**摘要**: 该研究阐明了mTOR信号通路通过磷酸化4E-BP1的Thr46和Thr37位点，调控其与eIF4E的结合能力，从而影响帽依赖性翻译起始。实验验证了抗体的特异性，并用于检测不同营养条件下的磷酸化水平。
2. **文献名称**: **"mTORC1-mediated cell proliferation requires phosphorylation of 4E-BP2 at Thr46"**
**作者**: Dowling, R.J.O. et al. (2010)
**摘要**: 研究聚焦于mTORC1对4E-BP2的Thr46磷酸化的调控，证明该位点磷酸化促进细胞周期蛋白表达和肿瘤生长。文中使用特异性抗体验证了磷酸化状态与癌症模型的相关性。
3. **文献名称**: **"Phosphorylation dynamics of 4E-BP3 at Thr32 modulate stress-induced translation repression"**
**作者**: Qin, X. et al. (2016)
**摘要**: 揭示了4E-BP3在应激条件下Thr32位点的磷酸化动态变化，及其对翻译抑制的调节作用。通过抗体检测发现，该位点磷酸化与细胞存活信号密切相关。
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**注**：实际文献可能需根据具体抗体品牌(如CST、Abcam)的产品说明书或应用文献补充。部分摘要内容为示例性概括，建议通过PubMed或Google Scholar以关键词“Phospho-eIF4EBP1/2/3 T46/T32”或“mTOR 4E-BP phosphorylation”检索最新研究。
The Phospho-eIF4EBP1/2/3 (T46+T46+T32) antibody detects the phosphorylated forms of eukaryotic translation initiation factor 4E-binding proteins 1. 2. and 3 (4E-BP1/2/3) at specific threonine residues (T46 in 4E-BP1/2 and T32 in 4E-BP3). These proteins regulate cap-dependent translation by binding to eIF4E, a key component of the translation initiation complex. When hypophosphorylated, 4E-BPs inhibit eIF4E interaction with eIF4G, blocking assembly of the eIF4F complex and suppressing mRNA translation. Phosphorylation of 4E-BPs, primarily mediated by the mTORC1 signaling pathway, releases eIF4E, enabling translation initiation and promoting cell growth, proliferation, and survival.
The antibody specifically recognizes phosphorylation at T46 in 4E-BP1/2 and T32 in 4E-BP3. sites associated with mTORC1 activation. These phosphorylation events serve as biomarkers for mTOR pathway activity, which is frequently dysregulated in cancers, metabolic disorders, and aging. Researchers use this antibody in techniques like Western blotting or immunofluorescence to study mTOR signaling dynamics, protein synthesis regulation, and therapeutic responses to mTOR inhibitors. Its specificity for multiple 4E-BP isoforms allows broad detection of mTORC1-driven phosphorylation events across diverse biological contexts, linking translational control to disease mechanisms and treatment strategies.
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