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产品描述：人粘蛋白2(MUC2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的MUC2含量。MUC2是一种黏蛋白，在人体胃肠道等黏膜表面广泛存在，形成黏液层，保护上皮细胞。研究显示其表达异常与肠道炎症、肿瘤等疾病密切相关。机制上，它可通过调节黏液屏障功能、影响细胞信号传导等参与疾病发生发展过程，是潜在治疗靶点。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于科研领域探究胃肠道疾病机制、评估细胞或组织模型中粘液层功能变化、筛选调控MUC2表达的化合物等研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Intestinal mucin 2 ELISA Kit; Intestinal mucin-2 ELISA Kit; MLP ELISA Kit; MUC 2 ELISA Kit; MUC-2 ELISA Kit; Muc2 ELISA Kit; MUC2_HUMAN ELISA Kit; Mucin 2 ELISA Kit; Mucin 2 intestinal ELISA Kit; Mucin 2 intestinal/tracheal ELISA Kit; Mucin 2 oligomeric mucus/gel forming ELISA Kit; Mucin 2 precursor ELISA Kit; Mucin 2 tracheal ELISA Kit; Mucin like protein ELISA Kit; Mucin-2 ELISA Kit; Mucin2 ELISA Kit; SMUC ELISA Kit
缩写：MUC2
Uniprot No.：Q02817
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MUC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 99-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of human MUC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-88 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.5312.4812.5062.3641002.0321.9611.9971.855501.4481.4011.4251.283250.8760.8230.8500.70812.50.5970.6230.6100.4686.250.3910.4020.3970.2553.120.2330.2170.2250.08300.1440.1390.142 | ||||||
本试剂盒所含材料：
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A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MUC2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled MUC2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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