| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | cAMP dependent protein kinase catalytic subunit alpha; cAMP dependent protein kinase catalytic subunit beta; cAMP dependent protein kinase catalytic subunit gamma; PKA C alpha; PKA C beta; PKA C gamma; PKACA; PRKACA; PRKACB; PRKACG;;p-PKA C alpha/beta/gamma (T197) |
| WB Predicted band size | Calculated MW: 40,41 kDa ; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PKA C alpha around the phosphorylation site of T197 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于Phospho-PKAC(Thr197)抗体的3篇参考文献示例(内容为虚构，仅供参考)：
1. **文献名称**: "Phosphorylation of Thr197 in PKACα is essential for its kinase activity and cellular localization"
**作者**: Smith A, et al.
**摘要**: 本研究通过点突变和体外激酶实验，证明PKACα Thr197位点的磷酸化是其催化活性的关键调节因素，并利用Phospho-PKAC(Thr197)抗体验证了该位点在细胞内的动态修饰与亚细胞定位的关系。
2. **文献名称**: "A cardiac-specific role of PKACβ phosphorylation at Thr197 in heart failure progression"
**作者**: Zhang Y, et al.
**摘要**: 通过构建心肌细胞特异性敲除模型，结合Phospho-PKAC(Thr197)抗体的免疫印迹分析，揭示了Thr197磷酸化水平与心衰病理中cAMP/PKA信号异常激活的关联性。
3. **文献名称**: "Development and validation of a phospho-specific antibody targeting PKAC Thr197 for cancer signaling studies"
**作者**: Taylor R, et al.
**摘要**: 本文报道了一种高特异性Phospho-PKAC(Thr197)抗体的开发流程，并通过肽竞争实验和磷酸酶处理验证其特异性，进一步应用于多种癌细胞系中PKA活性调控的研究。
注：以上文献为示例，实际文献需通过PubMed等数据库检索确认。
**Background of Phospho-PKAC(Thr197) Antibody**
The Phospho-PKAC(Thr197) antibody is a specialized tool used to detect the activated form of the catalytic subunit of protein kinase A (PKA), a critical enzyme in cAMP-mediated signaling. PKA consists of two regulatory (R) and two catalytic (C) subunits. Upon cAMP binding, the R subunits dissociate, releasing active C subunits (PKAC) to phosphorylate downstream targets.
Threonine 197 (Thr197) in PKAC is located within the activation loop, a region essential for enzymatic activity. Autophosphorylation at Thr197 is required for full PKA activation, stabilizing the active conformation and enhancing substrate binding. This post-translational modification serves as a biomarker for PKA activation in cellular responses to hormones, neurotransmitters, and stress signals.
The Phospho-PKAC(Thr197) antibody specifically recognizes PKAC when phosphorylated at Thr197. enabling researchers to study PKA activation dynamics in various contexts, including metabolic regulation, cell proliferation, and apoptosis. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess PKA activity in diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions.
By targeting this phosphorylation event, the antibody provides insights into cAMP/PKA pathway dysregulation and aids in evaluating therapeutic interventions targeting PKA signaling. Its specificity and reliability make it a valuable tool in both basic research and clinical studies.
×
