首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胃动素(MTL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MTL含量。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，该产品适用于科研领域中对胃肠动力调控机制、消化系统疾病模型研究或药物干预效果的评估，例如通过检测不同生理或病理状态下MTL的动态变化，探究其与胃肠功能障碍的关联性。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：MTL
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MTL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 86-95 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 94-104 | |||||
回收率：
| The recovery of human MTL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 81-92 | ||||
| EDTA plasma (n=4) | 94 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12002.3402.2722.3062.1846001.8611.7721.8171.6953001.2111.2661.2391.1171500.6930.7260.7100.588750.4370.4730.4550.33337.50.3020.2790.2910.16918.750.2130.2100.2120.09000.1240.1190.122 | ||||||
货期：3-5 working days
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