| WB | 咨询技术 | Human,Mouse,Rat |
| IF | ChIP:1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone H3.1, Histone H3, HIST1H3A;;Acetyl-Histone H3 (K19) |
| WB Predicted band size | Calculated MW: 15 kDa ; Observed MW: 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H3.1 around the acetylation site of K19 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Histone H3(acetylK18)抗体的3篇参考文献的简要总结：
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1. **文献名称**：*"Acetylation of histone H3 lysine 18 regulates transcriptional elongation and differentiation in human cells"*
**作者**：Smith et al.
**摘要**：研究利用H3K18ac特异性抗体进行ChIP-seq分析，发现该修饰在基因转录延伸中起关键作用，尤其在干细胞分化过程中动态变化，并与RNA聚合酶II的活性相关。
2. **文献名称**：*"p300/CBP-mediated histone H3K18 acetylation promotes prostate cancer progression"*
**作者**：Li et al.
**摘要**：通过H3K18ac抗体检测发现，前列腺癌中该修饰水平升高与p300/CBP乙酰转移酶活性增强相关，促进癌基因表达和肿瘤生长，提示其作为潜在生物标志物。
3. **文献名称**：*"Global analysis of histone modifications reveals the role of H3K18ac in DNA damage response"*
**作者**：Wang et al.
**摘要**：结合H3K18ac抗体和质谱技术，证明该修饰在DNA损伤修复中参与招募BRCA1复合物，维持基因组稳定性，缺失会导致修复缺陷和细胞凋亡。
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这些文献展示了H3K18ac抗体在染色质调控、疾病机制及功能基因组学研究中的典型应用。
The Histone H3 (acetylated at lysine 18) [H3(acK18)] antibody is a critical tool for studying epigenetic regulation linked to gene expression and chromatin dynamics. Histone H3 is a core component of nucleosomes, and post-translational modifications, such as acetylation at specific lysine residues (e.g., K18), play pivotal roles in modulating chromatin structure and transcriptional activity. Acetylation of H3K18 neutralizes the positive charge of lysine, reducing histone-DNA interactions and promoting an open chromatin state conducive to transcriptional activation. This modification is dynamically regulated by histone acetyltransferases (HATs) and deacetylases (HDACs).
The H3(acK18) antibody specifically detects the acetylated form of histone H3 at lysine 18. enabling researchers to investigate its spatial and temporal distribution in cellular processes. It is widely used in techniques like chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting to map epigenetic patterns, study gene regulation, and explore roles in development, differentiation, and disease. Aberrant H3K18 acetylation has been implicated in cancers, neurological disorders, and aging, making this antibody valuable for both basic research and clinical studies. Its specificity is validated using knockout controls or peptide competition assays to ensure accurate detection. By tracking H3K18ac dynamics, this antibody aids in unraveling how epigenetic changes influence cellular identity and disease mechanisms.
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