| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | C-TAK1; cTAK1; Emk2; KP78; Mark3; PAR1A;;MARK3 |
| WB Predicted band size | Calculated MW: 84 kDa ; Observed MW: 86 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human MARK3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于MARK3抗体的3篇参考文献，包含文献名称、作者及摘要概述：
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1. **文献名称**: *"MARK3/Par-1 kinase is required for microtubule stabilization and polarity determination in Drosophila oocytes"*
**作者**: Timm T., et al. (2003)
**摘要**: 研究利用MARK3抗体验证其在果蝇卵母细胞中的定位，证明MARK3通过磷酸化微管相关蛋白调控细胞极性及微管稳定性，对胚胎发育至关重要。
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2. **文献名称**: *"The PAR-1/MARK family of protein kinases: from polarity to metabolism"*
**作者**: Drewes G., et al. (2012)
**摘要**: 综述MARK家族(含MARK3)在细胞极性、能量代谢及神经退行性疾病中的作用，强调MARK3抗体在检测其与Tau蛋白互作及磷酸化修饰中的应用。
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3. **文献名称**: *"MARK3 mediates tumor progression through regulating AMPK-related signaling in colorectal cancer"*
**作者**: Li Y., et al. (2018)
**摘要**: 通过MARK3抗体敲低实验，揭示MARK3通过调控AMPK/mTOR通路促进结直肠癌增殖和转移，提示其作为潜在治疗靶点。
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4. **文献名称**: *"Structural basis of MARK3 kinase autoregulation by its spacer region"*
**作者**: Marx A., et al. (2020)
**摘要**: 利用MARK3抗体结合X射线晶体学，解析MARK3激酶结构域自抑制机制，阐明其活性调控对细胞迁移的影响。
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以上研究均通过MARK3抗体实现蛋白检测或功能干预，涵盖发育生物学、癌症及结构机制等领域。
MARK3 (microtubule affinity-regulating kinase 3), also known as C-TAK1 or PAR1A, is a serine/threonine kinase belonging to the AMPK-related kinase family. It plays a critical role in regulating cell polarity, microtubule dynamics, and signal transduction by phosphorylating microtubule-associated proteins such as Tau and MAP2. MARK3 is involved in diverse cellular processes, including cell cycle progression, migration, and apoptosis, through interactions with pathways like Wnt, mTOR, and MAPK. Its activity is modulated by phosphorylation and binding partners like 14-3-3 proteins.
Research links MARK3 dysregulation to several diseases. Overexpression or aberrant activation of MARK3 has been observed in cancers, where it may promote tumor growth and metastasis. In neurodegenerative disorders like Alzheimer’s disease, MARK3-mediated Tau hyperphosphorylation contributes to neurofibrillary tangle formation. Additionally, MARK3 polymorphisms are associated with metabolic syndromes and immune disorders.
MARK3 antibodies are essential tools for studying these mechanisms. They enable detection of MARK3 expression levels, phosphorylation status, and localization in tissues or cultured cells via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Commercial MARK3 antibodies are typically validated for specificity against unique epitopes, though isoform cross-reactivity remains a consideration due to homology within the MARK family. These reagents support ongoing investigations into MARK3’s pathophysiological roles and therapeutic targeting potential.
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