首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人金属硫蛋白2(MT-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MT2A含量。MT2A 即金属硫蛋白 2A，在生物体中广泛存在。它能结合多种金属离子，参与体内重金属解毒、维持金属离子稳态。其基因表达受金属、激素等诱导调节。研究发现，MT2A 还与肿瘤发生发展、细胞应激反应等相关机制存在联系。试剂盒检测范围为0.312 ng/mL-20 ng/mL，可广泛应用于基础研究领域，如探究重金属代谢机制、氧化应激相关疾病模型中的MT-2动态表达，或评估环境毒理学实验中生物标志物的响应规律，为金属硫蛋白家族的功能研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MT2A ELISA Kit; CES1 ELISA Kit; MT2 ELISA Kit; Metallothionein-2 ELISA Kit; MT-2 ELISA Kit; Metallothionein-2A ELISA Kit; Metallothionein-II ELISA Kit; MT-II ELISA Kit
缩写：MT2A
Uniprot No.：P02795
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MT-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of human MT-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-97 | ||||
| EDTA plasma (n=4) | 95 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.3212.4122.3672.215101.9381.9561.9471.79551.4431.3341.3891.2372.50.9210.9340.9280.7761.250.5010.4770.4890.3370.6250.3420.3380.3400.1880.3120.2540.2420.2480.09600.1530.1510.152 | ||||||
货期：3-5 working days
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