首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人髓鞘蛋白P0(MPZ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MPZ含量。MPZ是重要的靶点。它是髓鞘蛋白零，在周围神经髓鞘形成与维持中起关键作用。研究聚焦于其在神经脱髓鞘疾病中的机制，如通过影响髓鞘稳定性致病，探索其与疾病发生发展的关系，有望为相关疾病防治提供新策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品可应用于基础医学研究中周围神经疾病发病机制的探索、药物对髓鞘再生影响的评估，或用于筛选体外模型中MPZ的表达水平变化。通过优化实验流程，试剂盒支持科研人员高效完成多种生物样本的批量检测，为神经生物学、病理学及药理学研究提供可靠的技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Charcot Marie Tooth neuropathy 1B ELISA Kit; CHM ELISA Kit; CMT1 ELISA Kit; CMT1B ELISA Kit; CMT2I ELISA Kit; CMT2J ELISA Kit; CMT4E ELISA Kit; CMTDI3 ELISA Kit; CMTDID ELISA Kit; DSS ELISA Kit; HMSNIB ELISA Kit; MPP ELISA Kit; MPZ ELISA Kit; Myelin peripheral protein ELISA Kit; Myelin protein P0 ELISA Kit; Myelin protein zero ELISA Kit; MYP0_HUMAN ELISA Kit; P0 ELISA Kit
缩写：MPZ
Uniprot No.：P25189
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MPZ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 90-97 | ||||||
回收率：
| The recovery of human MPZ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 106 | 101-112 | |||||
| EDTA plasma (n=4) | 97 | 92-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.4932.5162.5052.37610002.2842.1012.1932.0645001.7151.7531.7341.6052501.2711.2431.2571.1281250.8490.8120.8310.70262.50.5420.5790.5610.43231.250.3270.3440.3360.20700.1300.1270.129 | |||||||
货期：3-5 working days
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