首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人KI-67抗原(Ki-67)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的KI-67含量。KI-67是一种与细胞增殖相关的核抗原。其背景在于可作为细胞增殖的标记物。研究机制方面，它在细胞增殖周期的G1、S、G2和M期表达，而在G0期不表达，因此检测其表达水平有助于了解细胞增殖活性，辅助肿瘤等疾病诊断和预后评估。试剂盒检测范围为0.312 ng/mL-20 ng/mL，肿瘤生物学研究、细胞增殖能力评估、抗肿瘤药物作用机制探索、肿瘤模型研究、药物干预后细胞增殖抑制效果评价、临床前研究中肿瘤标志物水平变化的分析、体外实验中对细胞或组织样本增殖活性的动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Antigen identified by monoclonal ELISA Kit Ki 67 ELISA Kit; Antigen identified by monoclonal ELISA Kit Ki-67 ELISA Kit; Antigen KI-67 ELISA Kit; Antigen KI67 ELISA Kit; Antigen Ki67 ELISA Kit; KI67_HUMAN ELISA Kit; KIA ELISA Kit; Marker of proliferation Ki-67 ELISA Kit; MIB 1 ELISA Kit; MIB ELISA Kit; MKI67 ELISA Kit; PPP1R105 ELISA Kit; Proliferation marker protein Ki-67 ELISA Kit; Proliferation related Ki 67 antigen ELISA Kit; Protein phosphatase 1 regulatory subunit 105 ELISA Kit; RP11-380J17.2 ELISA Kit
缩写：MKI67
Uniprot No.：P46013
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Ki-67 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 86-106 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 93-107 | |||||
回收率：
| The recovery of human Ki-67 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-106 | ||||
| EDTA plasma (n=4) | 99 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.2432.2812.2622.070101.6441.6511.6481.45651.1771.2711.2241.0322.50.8740.8990.8870.6951.250.6130.6270.6200.4280.6250.4680.4780.4730.2810.3120.3730.3890.3810.18900.1910.1930.192 | ||||||
货期：3-5 working days
×
