首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Ⅰ类MHC多肽相关序列B(MICB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MICB含量。MICB 是重要的免疫相关靶点，在机体免疫监视中起关键作用。它可被自然杀伤细胞、细胞毒性 T 淋巴细胞识别。研究聚焦其与肿瘤免疫逃逸关系，探索通过调控 MICB 表达和功能激活免疫细胞杀伤肿瘤，为肿瘤免疫治疗提供新思路。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本试剂盒适用于科研领域中对MICB蛋白的定量分析，例如探究感染性疾病中宿主细胞应激信号、肿瘤微环境免疫调控机制，或评估实验模型中细胞应激相关通路活性，为免疫学、肿瘤学及炎症相关研究的生物标志物探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MHC class I chain related protein B ELISA Kit; MHC class I chain related protein B2 ELISA Kit; MHC class I like molecule PERB11.2 IMX ELISA Kit; MHC class I mic B antigen ELISA Kit; MHC class I polypeptide related sequence B ELISA Kit; MHC class I polypeptide-related sequence B ELISA Kit; MIC B ELISA Kit; MIC-B ELISA Kit; MICB ELISA Kit; MICB_HUMAN ELISA Kit; PERB11.2 ELISA Kit; Stress inducible class I homolog ELISA Kit
缩写：MICB
Uniprot No.：Q29980
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MICB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 82-95 | ||||||
| 1:2 | Average % | 99 | |||||
| Range % | 93-104 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 89-99 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 88-98 | ||||||
回收率：
| The recovery of human MICB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 94-101 | |||||
| EDTA plasma (n=4) | 86 | 82-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 20002.3642.4772.4212.267 10001.7671.6541.7111.557 5001.0811.1361.1090.955 2500.7130.7240.7190.565 1250.4420.4550.4490.295 62.50.3710.3670.3690.215 31.250.2520.2590.2560.102 00.1530.1540.154 | |||||||
货期：3-5 working days
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