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产品描述：人巨噬细胞炎性蛋白2(MIP-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液样本中的MIP-2含量。MIP-2（巨噬细胞炎症蛋白-2）是一种CXC趋化细胞因子，主要由活化的单核细胞和中性粒细胞产生，并在炎症部位表达。它对中性粒细胞具有趋化和活化作用，通过其受体CXCR2促进白细胞向炎症部位迁移，参与多种免疫反应，如伤口愈合、癌症转移和血管生成。MIP-2在急性胰腺炎肺损伤中发挥重要作用，可能通过介导白细胞的趋化和活化在病理过程中发挥作用。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于体外炎症机制研究、免疫调控实验及疾病模型中的生物标志物分析，例如评估细胞因子释放水平、药物干预效果或病理状态下炎症通路激活程度。适用于生命科学基础研究与生物医药开发领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C-X-C motif chemokine 2 ELISA Kit; Chemokine (C X C motif) ligand 2 ELISA Kit; Chemokine; CXC motif; ligand 2 ELISA Kit; CINC 2a ELISA Kit; CINC2a ELISA Kit; CINC3 ELISA Kit; CXC chemokine ELISA Kit; CXCL 2 ELISA Kit; Cxcl2 ELISA Kit; CXCL2_HUMAN ELISA Kit; Cytokine-induced neutrophil chemoattractant 3 ELISA Kit; GRO 2 ELISA Kit; GRO b ELISA Kit; GRO protein; beta ELISA Kit; Gro-beta ELISA Kit; GRO-beta(5-73) ELISA Kit; GRO-beta-T ELISA Kit; GRO2 ELISA Kit; GRO2 oncogene ELISA Kit; GROb ELISA Kit; GRObeta ELISA Kit; Growth regulated protein beta ELISA Kit; Growth-regulated protein beta ELISA Kit; GROX ELISA Kit; Hematopoietic synergistic factor ELISA Kit; HSF ELISA Kit; Macrophage inflammatory protein 2 alpha ELISA Kit; Macrophage inflammatory protein 2 ELISA Kit; Macrophage inflammatory protein 2-alpha ELISA Kit; Melanoma growth stimulatory activity beta ELISA Kit; MGSA b ELISA Kit; MGSA beta ELISA Kit; MIP 2 ELISA Kit; MIP 2a ELISA Kit; MIP2 alpha ELISA Kit; MIP2 ELISA Kit; MIP2-alpha ELISA Kit; MIP2A ELISA Kit; MIP2alpha ELISA Kit; SB-251353 ELISA Kit; SCYB 2 ELISA Kit; Scyb ELISA Kit; SCYB2 ELISA Kit; Small inducible cytokine subfamily B; member 2 ELISA Kit
缩写：MIP-2
Uniprot No.：P19875
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, urine
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-100 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 95-106 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human MIP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 82 | 80-85 | ||||
| EDTA plasma (n=4) | 89 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.3522.4522.4022.2761001.8121.8191.8161.690501.1741.2731.2241.098250.7030.7230.7130.58712.50.4250.4280.4270.3016.250.3010.3210.3110.1853.1250.2010.2140.2080.08200.1250.1270.126 | ||||||
货期：3-5 working days
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