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产品描述：人肌醇加氧酶(MIOX)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MIOX含量。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于研究肌醇代谢相关疾病的分子机制、评估细胞或组织模型中MIOX表达调控，以及探索氧化应激状态下酶活性变化的科研实验。实验仅需100μL样本即可完成检测，配套提供标准曲线绘制与样本浓度计算方法，满足体外实验中对微量样本的高精度定量需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：0610009I10Rik ELISA Kit; AI314022 ELISA Kit; Aldehyde reductase-like 6 ELISA Kit; ALDRL6 ELISA Kit; C85427 ELISA Kit; Inositol oxygenase ELISA Kit; Kidney-specific protein 32 ELISA Kit; KSP32 ELISA Kit; MGC90217 ELISA Kit; MI oxygenase ELISA Kit; miox ELISA Kit; MIOX_HUMAN ELISA Kit; Myo-inositol oxygenase ELISA Kit; Renal-specific oxidoreductase ELISA Kit; RSOR ELISA Kit
缩写：MIOX
Uniprot No.：Q9UGB7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MIOX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 84-92 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 99-105 | ||||||
| 1:4 | Average % | 86 | |||||
| Range % | 81-90 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
回收率：
| The recovery of human MIOX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 89-100 | |||||
| EDTA plasma (n=4) | 91 | 88-95 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected12002.4432.3782.4112.2176002.0452.0562.0511.8573001.5771.3981.4881.2941500.9840.9870.9860.792750.6410.6380.6400.44637.50.4340.4450.4400.24618.750.3340.3290.3320.13800.1950.1930.194 | |||||||
货期：3-5 working days
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