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产品描述：人乳糖凝集素8(LGALS8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LGALS8含量。LGALS8 即半乳糖凝集素 -8，是一种内源性凝集素。它参与多种生理病理过程，如免疫调节、细胞黏附等。研究机制上，其可识别受损细胞表面糖基化变化，激活自噬清除病原体。在肿瘤等疾病研究中，被关注作为潜在治疗靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于基础科研领域，如探究LGALS8在炎症反应、肿瘤进展或微生物感染模型中的动态表达规律，或通过检测不同处理条件下生物样本中LGALS8的浓度变化，解析其分子调控机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gal 8 ELISA Kit; Gal-8 ELISA Kit; Gal8 ELISA Kit; Galectin 8 ELISA Kit; Galectin-8 ELISA Kit; galectin-8g ELISA Kit; Lectin galactoside binding soluble 8 ELISA Kit; LEG8_HUMAN ELISA Kit; LGAL S8 ELISA Kit; Lgals8 ELISA Kit; PCTA 1 ELISA Kit; PCTA-1 ELISA Kit; PCTA1 ELISA Kit; Po66 carbohydrate binding protein ELISA Kit; Po66 carbohydrate-binding protein ELISA Kit; Po66 CBP ELISA Kit; Po66-CBP ELISA Kit; Prostate carcinoma tumor antigen 1 ELISA Kit
缩写：LGALS8
Uniprot No.：O00214
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LGALS8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 87-99 | ||||||
| 1:2 | Average % | 87 | |||||
| Range % | 83-92 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 84-93 | ||||||
| 1:8 | Average % | 99 | |||||
| Range % | 91-103 | ||||||
回收率：
| The recovery of human LGALS8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 81-93 | |||||
| EDTA plasma (n=4) | 95 | 88-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 20002.0121.9861.9991.826 10001.5971.5351.5661.393 5001.1061.0671.0870.914 2500.8330.8020.8180.645 1250.5210.5140.5180.345 62.50.3840.3790.3820.209 31.250.2790.2720.2760.103 00.1750.1710.173 | |||||||
货期：3-5 working days
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