| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Grp170; HSP12A; Hyou1; Orp150;;HYOU1 |
| WB Predicted band size | Calculated MW: 111 kDa ; Observed MW: 150 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human HYOU1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于ORP150抗体的参考文献示例(注：以下内容为基于领域知识的模拟示例，具体文献需通过学术数据库验证)：
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1. **文献名称**: *Oxygen-regulated protein 150 (ORP150) suppresses hypoxia-induced apoptotic cell death in brain neurons*
**作者**: Ozawa K et al.
**摘要**: 本研究利用ORP150抗体，通过免疫组化和Western blot分析，发现脑缺血后神经元中ORP150表达显著上调。实验表明ORP150通过抑制内质网应激介导的凋亡通路，发挥神经保护作用。
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2. **文献名称**: *Role of ORP150 in diabetic nephropathy: Insights from antibody-based inhibition studies*
**作者**: Miyazaki M et al.
**摘要**: 作者通过注射ORP150中和抗体，发现其可加剧糖尿病模型小鼠的肾小球损伤，提示ORP150在缓解高糖诱导的内质网应激及肾细胞损伤中起关键作用。
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3. **文献名称**: *ORP150 as a prognostic biomarker in glioblastoma: Immunohistochemical analysis*
**作者**: Tamatani T et al.
**摘要**: 利用ORP150抗体对胶质母细胞瘤组织进行染色，发现高表达ORP150与患者生存期延长相关，提示其可能通过调节肿瘤微环境缺氧反应影响预后。
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4. **文献名称**: *ORP150 modulates macrophage lipid metabolism in atherosclerosis*
**作者**: Bando Y et al.
**摘要**: 研究通过ORP150抗体阻断实验，揭示其在巨噬细胞内质网中的脂质代谢调控作用，ORP150缺失促进泡沫细胞形成，加剧动脉粥样硬化斑块发展。
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建议通过 **PubMed** 或 **Google Scholar** 检索具体文献，关键词："ORP150 antibody"、"HYOU1 antibody" 或结合研究领域(如"ORP150 cancer")。部分研究可能涉及抗体在疾病机制、诊断或治疗中的应用。
The ORP150 antibody targets the oxygen-regulated protein 150 (ORP150), also known as GRP170 or HYOU1. a 150 kDa molecular chaperone belonging to the heat shock protein 70 (HSP70) family. ORP150 is primarily localized in the endoplasmic reticulum (ER) and plays a critical role in cellular stress responses, particularly under hypoxic conditions or ER stress. It facilitates protein folding, degradation of misfolded proteins, and maintains ER homeostasis, making it vital for cell survival during oxygen deprivation, ischemia, or inflammatory challenges. Its expression is upregulated in pathological conditions like cancer, neurodegenerative diseases, and cardiovascular disorders, where cellular stress is prominent.
ORP150 antibodies are widely used in research to study its expression, localization, and function in both normal and diseased tissues. These antibodies enable detection via techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Monoclonal and polyclonal variants are available, often validated for specificity across human, mouse, and rat models. Researchers utilize ORP150 antibodies to explore its role as a biomarker for ER stress and hypoxia in tumor microenvironments or ischemic injuries. Additionally, studies focus on its therapeutic potential, as modulating ORP150 activity may influence disease progression. Proper validation of antibody specificity, including knockout controls, is essential due to its structural similarity to other HSP70 members. Overall, ORP150 antibodies are key tools in understanding cellular stress mechanisms and developing targeted therapies.
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