| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RCC1; RCC1-I; SNHG3-RCC1; CHC1;;RCC1 |
| WB Predicted band size | 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human RCC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于RCC1抗体的3篇参考文献，按文献名称、作者和摘要内容简要概括：
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1. **"RCC1 functions as a regulator of chromosomal condensation dynamics during mitosis"**
*作者：Nishimoto T. et al.*
**摘要**：研究通过RCC1抗体的免疫荧光和Western blot技术，揭示了RCC1蛋白在有丝分裂期间调控染色体凝聚动态的机制，并发现其与Ran GTP酶的相互作用对核膜重组至关重要。
2. **"The role of RCC1 in maintaining genomic stability: Insights from antibody-based knockdown experiments"**
*作者：Clarke P.R. et al.*
**摘要**：利用RCC1特异性抗体进行免疫沉淀和功能抑制实验，证明RCC1通过调控染色质结构维持基因组稳定性，其缺失会导致DNA损伤修复缺陷和细胞周期停滞。
3. **"Development and validation of a novel RCC1 monoclonal antibody for cancer biomarker studies"**
*作者：Zhang L. et al.*
**摘要**：报道了一种高特异性RCC1单克隆抗体的开发，并通过免疫组化在多种癌症组织中验证其应用，结果显示RCC1表达水平与肿瘤侵袭性及预后相关。
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以上文献均涉及RCC1抗体的实验应用(如Western blot、免疫荧光、免疫组化等)，并聚焦于其在细胞周期、基因组稳定性及癌症中的功能研究。如需具体发表年份或期刊信息，可进一步补充检索。
RCC1 (Regulator of Chromosome Condensation 1) is a conserved protein critical for cell cycle regulation, primarily functioning as the guanine nucleotide exchange factor (GEF) for the small GTPase Ran. It facilitates the exchange of GDP for GTP on Ran, generating a RanGTP gradient essential for nucleocytoplasmic transport, mitotic spindle assembly, and nuclear envelope formation. RCC1 binds chromatin through its seven-bladed β-propeller structure, interacting with histones H2A/H2B and DNA, ensuring its localization during mitosis. Dysregulation of RCC1 is linked to genomic instability and cancer progression.
RCC1 antibodies are immunological tools used to detect and study RCC1 expression, localization, and function in various biological contexts. These antibodies are critical in research applications such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). They help elucidate RCC1's role in cell cycle checkpoints, chromatin organization, and its potential as a biomarker in cancers like renal cell carcinoma (RCC), where altered expression has been observed. Commercial RCC1 antibodies are typically raised in rabbits or mice against specific epitopes, with validation in knockout models to ensure specificity. Studies using these antibodies have advanced understanding of mitotic errors, aneuploidy, and cancer mechanisms, highlighting RCC1 as a therapeutic target in oncology.
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