首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人载脂运载蛋白-1(LCN1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、唾液样本中的LCN1含量。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于体外科研实验中LCN1蛋白水平的定量分析，例如探索干眼症发病机制、口腔黏膜屏障功能评估或脂质代谢相关疾病模型研究；为研究者提供高效工具以开展LCN1在生理病理过程中的功能研究及生物标志物筛选。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LCN1 ELISA Kit; LCN1_HUMAN ELISA Kit; Lipocalin 1 (protein migrating faster than albumin, tear prealbumin) ELISA Kit; Lipocalin 1 like 2 ELISA Kit; Lipocalin-1 ELISA Kit; PMFA ELISA Kit; Tear lipocalin ELISA Kit; Tear prealbumin ELISA Kit; Tlc ELISA Kit; TP ELISA Kit; VEG protein ELISA Kit; VEGP ELISA Kit; Von Ebner gland protein ELISA Kit
缩写：LCN1
Uniprot No.：P31025
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, saliva
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LCN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human LCN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 92 | 90-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.7142.7682.7412.654501.8121.8011.8071.720251.0051.0581.0320.94512.50.5380.5680.5530.4666.250.3010.3250.3130.2263.120.2450.2380.2420.1551.560.1420.1560.1490.06200.0880.0860.087 | ||||||
货期：3-5 working days
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