首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人细胞角蛋白20(CK20)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的KRT20含量。KRT20即角蛋白20，是一种上皮细胞角蛋白。它在胃肠道和泌尿道的上皮细胞中高度表达。研究发现其表达异常与多种肿瘤发生发展相关，比如肿瘤的增殖、侵袭与转移，可作为肿瘤诊断与预后评估的生物标志物，在肿瘤机制研究和治疗中有潜在价值。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该产品可用于体外研究肿瘤微环境中CK20的表达动态、评估肿瘤细胞分化程度、追踪消化道肿瘤动物模型生物标志物变化等科研场景，为肿瘤发生机制、药物干预效果及生物标志物筛选等研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD20 ELISA Kit; CK 20 ELISA Kit; CK-20 ELISA Kit; CK20 ELISA Kit; Cytokeratin-20 ELISA Kit; Cytokeratin20 ELISA Kit; K1C20_HUMAN ELISA Kit; K20 ELISA Kit; KA20 ELISA Kit; Keratin 20 ELISA Kit; keratin 20, type I ELISA Kit; keratin 21, rat, homolog of ELISA Kit; Keratin ELISA Kit; Keratin type I cytoskeletal 20 ELISA Kit; Keratin-20 ELISA Kit; Keratin20 ELISA Kit; KRT 20 ELISA Kit; KRT 21 ELISA Kit; KRT20 ELISA Kit; KRT21 ELISA Kit; MGC35423 ELISA Kit; OTTHUMP00000164518 ELISA Kit; Protein IT ELISA Kit; type I cytoskeletal 20 ELISA Kit
缩写：KRT20
Uniprot No.：P35900
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CK20 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 103 | ||||
| Range % | 96-108 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of human CK20 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-97 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.7662.8682.8172.673202.1011.9972.0491.905101.1721.2441.2081.06450.7250.7320.7290.5852.50.3630.3870.3750.2311.250.2660.2840.2750.1310.6250.2280.2460.2370.09300.1440.1430.144 | ||||||
货期：3-5 working days
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