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产品描述：人细胞角蛋白18(CK-18)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物、cell culturesupernates样本中的KRT18含量。KRT18，全称角蛋白18，是一种在非鳞状上皮细胞中表达的I型中间丝蛋白，参与细胞结构和细胞凋亡等过程。其研究机制涉及细胞凋亡、炎症、癌症和纤维化等信号通路，与多种疾病如肝硬化、椎间盘退变等密切相关。试剂盒检测范围为0.625 ng/mL-15 ng/mL，体外药物毒性评估、肿瘤微环境研究、组织损伤模型的科研、上皮相关疾病的分子机制探索、生物标志物筛选本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cell proliferation inducing gene 46 protein ELISA Kit; Cell proliferation inducing protein 46 ELISA Kit; Cell proliferation-inducing gene 46 protein ELISA Kit; CK 18 ELISA Kit; CK-18 ELISA Kit; CK18 ELISA Kit; CYK 18 ELISA Kit; CYK18 ELISA Kit; Cytokeratin 18 ELISA Kit; Cytokeratin endo B ELISA Kit; Cytokeratin-18 ELISA Kit; K 18 ELISA Kit; K18 ELISA Kit; K1C18_HUMAN ELISA Kit; KA18 ELISA Kit; Keratin 18 ELISA Kit; Keratin 18, type I ELISA Kit; Keratin D ELISA Kit; keratin, type I cytoskeletal 18 ELISA Kit; Keratin-18 ELISA Kit; Krt18 ELISA Kit
缩写：KRT18
Uniprot No.：P05783
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates, cell culture supernates
检测范围：0.625 ng/mL-15 ng/mL
灵敏度：0.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<10% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CK-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-99 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-106 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-103 | |||||
回收率：
| The recovery of human CK-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-102 | ||||
| EDTA plasma (n=4) | 97 | 92-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 151.9321.9581.9451.898 7.51.3181.3571.3381.291 3.750.7860.7980.7920.745 1.250.3750.3620.3690.322 0.6250.2160.2230.2200.173 00.0460.0470.047 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-glucagon antibody.
Six vials standard (5ml/bottle)--- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial HRP-conjugated glucagon antibody (6 ml/bottle) --- Bind to the glucagon in the samples or standards and react with the substrate to make the solution chromogenic.
One vial Wash Buffer (20x concentrate) (15 ml/bottle) --- Wash away unbound or free substances.
One vial Substrate A (7ml/bottle) --- Mix with substrate B and then interact with TMB to elicit a chromogenic reaction.
One vial Substrate B (7ml/bottle) --- Mix with substrate A and interact with TMB to elicit a chromogenic reaction.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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