| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hG4 1; Mpp1; p38 2G4; Pa2g4; Plfap;;PA2G4 |
| WB Predicted band size | Calculated MW: 44 kDa ; Observed MW: 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PA2G4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于EBP1/PA2G4抗体的3篇代表性文献及其摘要概括：
1. **文献名称**：*"PA2G4/EBP1 regulates cell proliferation and migration via interactions with the EGFR/MAPK pathway in breast cancer"*
**作者**：Zhang Y. et al.
**摘要**：本研究通过Western blot和免疫组化(使用EBP1特异性抗体)发现，EBP1在乳腺癌中高表达，并通过与EGFR/MAPK信号通路相互作用促进肿瘤细胞增殖和转移，提示其作为潜在治疗靶点。
2. **文献名称**：*"EBP1 interacts with ErbB3 to modulate apoptosis and cetuximab resistance in colorectal cancer"*
**作者**：Lee H. et al.
**摘要**：利用EBP1抗体进行免疫共沉淀实验，作者揭示了EBP1与ErbB3受体直接结合，抑制化疗药物诱导的结直肠癌细胞凋亡，并参与西妥昔单抗耐药性的分子机制。
3. **文献名称**：*"The role of PA2G4 in transcriptional regulation of androgen receptor signaling in prostate cancer"*
**作者**：Smith J. et al.
**摘要**：通过染色质免疫沉淀(ChIP)结合EBP1抗体，研究发现PA2G4作为雄激素受体(AR)共调控因子，促进前列腺癌中AR靶基因的转录，影响肿瘤生长和激素治疗反应。
4. **文献名称**：*"EBP1 knockdown suppresses leukemia cell survival via ribosomal RNA processing modulation"*
**作者**：Wang R. et al.
**摘要**：通过RNA干扰和EBP1抗体验证，该研究证实EBP1通过调控核糖体RNA加工维持白血病细胞存活，其缺失导致细胞周期阻滞和凋亡，为血液肿瘤治疗提供新方向。
以上文献均涉及EBP1/PA2G4抗体的实验应用，涵盖癌症机制、信号通路及治疗靶点等领域。
The EBP1/PA2G4 antibody targets the ErbB3-binding protein 1 (EBP1), also known as proliferation-associated protein 2G4 (PA2G4), a multifunctional protein involved in cell proliferation, differentiation, and apoptosis. EBP1 interacts with the ErbB3 receptor tyrosine kinase and modulates downstream signaling pathways, including RAS/ERK and PI3K/AKT, influencing cellular growth and survival. It exhibits dual roles in cancer, acting as either a tumor suppressor or promoter depending on cellular context, and is implicated in breast, prostate, and other cancers. EBP1 also participates in ribosome biogenesis, RNA metabolism, and transcriptional regulation, interacting with nuclear receptors and chromatin-modifying complexes.
Structurally, EBP1 has two isoforms, p48 and p42. generated by alternative splicing. The p42 isoform promotes cell survival, while p48 induces differentiation. EBP1/PA2G4 antibodies are essential tools for detecting these isoforms in research, aiding in studies of their distinct functions. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and interactions in both normal and pathological states. Commercial antibodies are typically raised in rabbits or mice, with clones like C1 or E1 validated for specificity. Research on EBP1/PA2G4 antibodies continues to explore its role in cancer progression, viral pathogenesis (e.g., interactions with EBV or HPV proteins), and neurodegenerative disorders, highlighting its therapeutic and diagnostic potential.
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