首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人转移抑制剂 KiSS-1(KISS1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的KISS1含量。KISS1 是重要靶点。它最初被发现与肿瘤转移抑制相关。其研究机制方面，KISS1 编码的蛋白可与受体结合，参与调节促性腺激素的分泌，在生殖内分泌调控中起关键作用，还可能通过影响细胞增殖、黏附等过程参与肿瘤进展。试剂盒检测范围为0.312 ng/mL-20 ng/mL，为肿瘤转移机制研究、生殖生理学探索以及代谢相关疾病的基础科研提供可靠工具，特别适用于体外实验模型中肿瘤生物学行为分析、激素调控网络研究及病理状态下蛋白表达动态监测等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：KISS 1 ELISA Kit; KiSS 1 metastasis suppressor ELISA Kit; Kiss1 ELISA Kit; KISS1_HUMAN ELISA Kit; Kisspeptin 1 ELISA Kit; Kisspeptin 10 ELISA Kit; Kisspeptin 13 ELISA Kit; Kisspeptin 14 ELISA Kit; Kisspeptin ELISA Kit; Kisspeptin-1 ELISA Kit; Kisspeptin-10 ELISA Kit; Kisspeptin-54 ELISA Kit; Kisspeptin1 ELISA Kit; Malignant melanoma metastasis suppressor ELISA Kit; Metastasis suppressor KiSS 1 ELISA Kit; Metastasis suppressor KiSS1 ELISA Kit; Metastin ELISA Kit; MGC39258 ELISA Kit
缩写：KISS1
Uniprot No.：Q15726
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KISS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-103 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of human KISS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 80-93 | ||||
| EDTA plasma (n=4) | 96 | 92-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.8422.9632.9032.775102.1872.2032.1952.06751.1481.2261.1871.0592.50.7550.7430.7490.6211.250.3490.3650.3570.2290.6250.2220.2050.2140.0860.3120.2020.2000.2010.07300.1320.1240.128 | ||||||
货期：3-5 working days
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