| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ddx17; P72; RH70;;DDX17 |
| WB Predicted band size | Calculated MW: 80 kDa ; Observed MW: 70,80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human DDX17 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于DDX17抗体的3篇代表性文献及其摘要概括：
1. **文献名称**：*"DDX17 interacts with the p53 tumor suppressor to regulate gene expression"*
**作者**：Moyano M, et al.
**摘要**：该研究通过免疫共沉淀(Co-IP)和染色质免疫沉淀(ChIP)结合DDX17抗体，揭示DDX17与p53直接互作，共同调控靶基因(如细胞周期相关基因)的表达，并参与抑制肿瘤发生。
2. **文献名称**：*"DDX17 is required for efficient viral RNA sensing and innate immunity activation"*
**作者**：Mersaoui SY, et al.
**摘要**：本文利用DDX17抗体进行RNA免疫沉淀(RIP)和蛋白质组学分析，发现DDX17通过识别病毒RNA并激活RIG-I/MAVS信号通路，在宿主抗病毒先天免疫应答中发挥关键作用。
3. **文献名称**：*"DDX17 maintains pluripotency by bridging super-enhancers in embryonic stem cells"*
**作者**：Liu X, et al.
**摘要**：研究通过ChIP-seq(使用DDX17抗体)和CRISPR筛选，证明DDX17通过结合超级增强子区域，维持胚胎干细胞多能性相关基因(如OCT4、NANOG)的转录活性。
4. **文献名称**：*"The DEAD-box protein DDX17: A multifunctional player in RNA metabolism"*
**作者**：Fuller-Pace FV.
**摘要**：综述性文章系统总结了DDX17在RNA剪切、核糖体生成、miRNA加工等过程中的功能，并强调了基于DDX17抗体的实验方法对其机制研究的贡献。
(注：以上文献为领域内典型研究方向示例，实际引用时建议通过PubMed或Google Scholar核对最新进展。)
The DEAD-box helicase DDX17 is a conserved RNA-binding protein involved in multiple aspects of RNA metabolism, including transcription, splicing, ribosome biogenesis, and miRNA processing. It plays critical roles in cellular processes such as cell cycle regulation, antiviral responses, and maintaining genomic stability. DDX17 antibodies are essential tools for studying its expression, localization, and molecular interactions in these pathways.
These antibodies are commonly used in techniques like Western blotting, immunoprecipitation (IP), and immunofluorescence (IF) to detect DDX17 in various biological samples. Due to DDX17's homology with other DEAD-box proteins (e.g., DDX5), antibody specificity is rigorously validated using knockout cell lines or siRNA-mediated knockdown to minimize cross-reactivity. Research applications focus on its role in cancer (e.g., acting as a tumor suppressor or promoter in context-dependent manner), neurodegeneration, and viral infection responses.
Commercial DDX17 antibodies are typically raised against specific epitopes within its N-terminal or C-terminal regions, often in hosts like rabbits or mice. Recent studies also highlight DDX17's phase separation activity and involvement in liquid-liquid phase separation (LLPS), driving the formation of membraneless organelles. Validated antibodies aid in exploring these emerging mechanisms, making them vital for dissecting DDX17's dual roles in RNA homeostasis and disease pathogenesis.
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