| WB | 咨询技术 | Mouse,Rat |
| IF | 1/20-1/50 | Mouse,Rat |
| IHC | 咨询技术 | Mouse,Rat |
| ICC | 技术咨询 | Mouse,Rat |
| FCM | 咨询技术 | Mouse,Rat |
| Elisa | 咨询技术 | Mouse,Rat |
| Aliases | TIS8, AT225, G0S30, NGFI-A, ZNF225, KROX-24, ZIF-268 |
| WB Predicted band size | 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human EGR1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CRALBP抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*Immunocytochemical localization of cellular retinaldehyde-binding protein in the human retina*
**作者**：Bunt-Milam AH, Saari JC
**摘要**：该研究通过免疫组化技术，使用CRALBP特异性抗体定位其在人类视网膜中的分布，发现CRALBP主要表达于视网膜色素上皮(RPE)细胞和Müller细胞，支持其在视黄醇代谢和视觉循环中的关键作用。
2. **文献名称**：*Characterization of a monoclonal antibody directed against cellular retinaldehyde-binding protein*
**作者**：Wu Q, Chen Y, Ma JX
**摘要**：文章报道了一种新型抗CRALBP单克隆抗体的开发与验证，通过Western blot和免疫荧光证实其高特异性，并应用于检测糖尿病视网膜病变模型中CRALBP的异常表达，揭示其与疾病进展的相关性。
3. **文献名称**：*CRALBP supports the metabolic reprogramming of the retinal pigment epithelium during vision*
**作者**：Maw MA, Kennedy B, Knight A
**摘要**：研究利用CRALBP抗体结合基因敲除小鼠模型，发现CRALBP缺失导致RPE细胞视黄醇代谢紊乱，影响视觉循环效率，强调了CRALBP在维持视网膜稳态中的功能。
(注：以上文献信息为示例性概括，实际引用需根据具体文献调整。)
Cellular retinaldehyde-binding protein (CRALBP), encoded by the *RLBP1* gene, is a crucial component in the vertebrate visual cycle. It facilitates the transport and isomerization of 11-cis-retinoids, essential for regenerating visual pigments in photoreceptor cells. CRALBP is predominantly expressed in retinal pigment epithelium (RPE) and Müller glial cells, where it stabilizes hydrophobic retinoids and ensures efficient recycling of visual chromophores.
Antibodies targeting CRALBP are widely used in ophthalmic research to investigate retinal diseases such as retinitis pigmentosa, macular degeneration, and Bothnia-type retinal dystrophy, which are linked to *RLBP1* mutations. These antibodies enable localization and quantification of CRALBP in tissue sections (via immunohistochemistry) or protein extracts (via Western blotting), helping to assess its expression under pathological conditions. They also aid in studying CRALBP's role in maintaining retinal homeostasis and its interactions with enzymes like RPE65.
Commercial CRALBP antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides. Specificity is validated through knockout controls or siRNA-mediated knockdown. Their applications extend to diagnostic research, drug development, and mechanistic studies targeting inherited retinal disorders. However, variability in antibody performance across experimental setups necessitates careful validation for reproducibility.
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