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Rabbit Monoclonal USP9x Antibody

  • 中文名： USP9x抗体
  • 别    名： DFFRX; FAF; Fafl; Fam; hFAM; MRX99; Usp9x; X chromosome; X-linked;;USP9X
货号: IPDX18148
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC IHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/20-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesDFFRX; FAF; Fafl; Fam; hFAM; MRX99; Usp9x; X chromosome; X-linked;;USP9X
WB Predicted band sizeCalculated MW: 290 kDa ; Observed MW: 292 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human USP9X
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是3篇关于USP9x抗体的参考文献(信息基于公开研究整理)：

1. **文献名称**：*USP9X deubiquitinates ALDH1A3 and maintains mesenchymal identity in glioblastoma stem cells*

**作者**：Huang et al.

**摘要**：该研究通过USP9x抗体免疫沉淀实验，揭示USP9x通过去泛素化ALDH1A3蛋白维持胶质母细胞瘤干细胞的间充质表型，促进肿瘤侵袭性。

2. **文献名称**：*USP9X interacts with SMAD4 and promotes TGF-β signaling in pancreatic cancer*

**作者**：Zhang et al.

**摘要**：利用USP9x特异性抗体进行蛋白质互作分析，发现USP9x与SMAD4结合并增强TGF-β信号通路，驱动胰腺癌转移。

3. **文献名称**：*USP9X regulates DNA damage-induced apoptosis via MCL1 stabilization in leukemia*

**作者**：Schoenfeld et al.

**摘要**：通过Western blot和免疫荧光(使用USP9x抗体)，证明USP9x通过稳定抗凋亡蛋白MCL1抑制白血病细胞DNA损伤后的凋亡，提示其作为治疗靶点。

(注：若需实际引用，请通过PubMed/Google Scholar核实具体文献信息及原文。)

背景信息

USP9x (Ubiquitin-Specific Protease 9 X-linked) is a deubiquitinating enzyme belonging to the USP family, which regulates protein stability by removing ubiquitin chains from substrate proteins. It plays critical roles in diverse cellular processes, including apoptosis, cell migration, DNA repair, and neuronal development. USP9x is implicated in various diseases, particularly cancer, where it can act as either an oncogene or tumor suppressor depending on context. For instance, it stabilizes oncoproteins like MCL-1 in some cancers but promotes degradation of pro-survival proteins in others. USP9x antibodies are essential tools for studying its expression, localization, and interactions in cells or tissues. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Researchers often validate USP9x antibodies using knockout cell lines or siRNA knockdown to confirm specificity. Commercially available antibodies typically target epitopes within its C-terminal catalytic domain or N-terminal regulatory regions. Species reactivity commonly includes human, mouse, and rat. Studies utilizing USP9x antibodies have uncovered its involvement in pathways like Wnt/β-catenin, Notch, and TGF-β signaling, as well as its role in epithelial-mesenchymal transition (EMT). Both monoclonal and polyclonal formats exist, with selection depending on application requirements. Proper antibody validation remains crucial due to USP9x’s structural homology with other USP family members.

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