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产品描述：人 KIAA1199酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CEMIP含量。CEMIP（细胞外基质金属蛋白酶诱导因子结合蛋白）在多种生物学过程中发挥作用。其研究机制显示它与细胞迁移、侵袭及基质重塑相关，在肿瘤发生发展里或扮演重要角色，会影响肿瘤的生长、转移等，是肿瘤治疗领域备受关注的潜在靶点。试剂盒检测范围为1 ng/mL-24 ng/mL，适用于基础医学研究领域，例如探究KIAA1199在肿瘤微环境调控、炎症反应或创伤修复中的作用机制，也可用于筛选与细胞迁移相关的药物靶点或评估疾病模型中的生物标志物表达变化，为肿瘤生物学和病理学研究提供重要工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CEMIP ELISA Kit; KIAA1199 ELISA Kit; Cell migration-inducing and hyaluronan-binding protein ELISA Kit; EC 3.2.1.35 ELISA Kit
缩写：KIAA1199
Uniprot No.：Q8WUJ3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1 ng/mL-24 ng/mL
灵敏度：0.4 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<10% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KIAA1199 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 99-103 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 81-92 | |||||
回收率：
| The recovery of human KIAA1199 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-98 | ||||
| EDTA plasma (n=4) | 104 | 98-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected242.5752.6342.6052.563121.4551.3741.4151.37360.7030.7180.7110.66920.2220.2350.2290.18710.1110.1160.1140.07200.0410.0420.042 | ||||||
货期：3-5 working days
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