首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人己酮糖激酶(KHK)酶联免疫试剂盒,为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的KHK含量。KHK即己酮糖激酶，是催化果糖代谢第一步反应的关键酶。研究显示其与代谢综合征等多种疾病密切相关。其研究机制主要聚焦于KHK对果糖代谢通路的调控，以及通过影响肝脏脂质合成等途径，在疾病发生发展中所起的作用。试剂盒检测范围为18.75 mU/mL-1200 mU/mL，适用于细胞能量代谢研究、动物模型代谢评估及体外药物干预实验等科研领域，可为果糖代谢相关分子机制探索、代谢综合征病理研究及潜在治疗靶点筛选提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EC 2.7.1.3 ELISA Kit; Fructokinase ELISA Kit; Hepatic fructokinase ELISA Kit; Ketohexokinase ELISA Kit; Ketohexokinase isoform a ELISA Kit; KHK ELISA Kit; KHK_HUMAN ELISA Kit
缩写：KHK
Uniprot No.：P50053
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：18.75 mU/mL-1200 mU/mL
灵敏度：4.68 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KHK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 84-94 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 93-103 | |||||
回收率：
| The recovery of human KHK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-102 | ||||
| EDTA plasma (n=4) | 90 | 86-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected12002.5472.4612.5042.3526002.1432.0762.1101.9583001.4031.4491.4261.2741500.8140.7650.7900.638750.3040.3250.3150.16337.50.2260.2180.2220.07018.750.1820.1690.1760.02400.1560.1470.152 | ||||||
货期：3-5 working days
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