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Rabbit Monoclonal APPL Antibody

  • 中文名: APPL抗体
  • 别    名: DIP13 alpha; DP13A; KIAA1428; APPL1; Dip13-alpha; Adapter protein containing PH domain; DCC-interacting protein 13-alpha;;Dip13 alpha
货号: IPDX18141
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC IHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesDIP13 alpha; DP13A; KIAA1428; APPL1; Dip13-alpha; Adapter protein containing PH domain; DCC-interacting protein 13-alpha;;Dip13 alpha
WB Predicted band sizeCalculated MW: 80 kDa ; Observed MW: 85 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human Dip13 alpha
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是3-4条关于APPL抗体的研究文献示例(内容基于公开研究概括,非真实文献,仅供示例参考):

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1. **文献名称**:**"APPL1 regulates insulin signaling through endosomal trafficking in diabetes"**

**作者**:Li et al.

**摘要**:研究APPL1蛋白在胰岛素信号通路中的作用,通过特异性抗体检测发现APPL1通过调控内体运输影响胰岛素受体活性,为2型糖尿病机制提供新见解。

2. **文献名称**:**"APPL2-mediated crosstalk between autophagy and apoptosis in cancer cells"**

**作者**:Wang & Chen

**摘要**:利用APPL2抗体揭示APPL2蛋白通过结合自噬相关蛋白LC3和凋亡因子BAX,在肿瘤细胞存活与死亡中的双向调控功能。

3. **文献名称**:**"APPL1 interacts with amyloid-beta precursor protein in Alzheimer's disease models"**

**作者**:Smith et al.

**摘要**:通过免疫共沉淀(使用APPL1抗体)证实APPL1与β-淀粉样蛋白前体(APP)在神经元中的相互作用,提示其可能参与阿尔茨海默病病理进程。

4. **文献名称**:**"Differential roles of APPL1 and APPL2 in adipocyte differentiation"**

**作者**:Zhang et al.

**摘要**:对比APPL1和APPL2抗体标记结果,发现APPL1促进脂肪细胞分化,而APPL2抑制该过程,揭示同源蛋白的功能差异性。

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如需真实文献,建议在PubMed或Google Scholar检索关键词:**"APPL1/APPL2 antibody" + "signaling"/"autophagy"/"diabetes"**。

背景信息

APPL (Adaptor Protein containing PH domain, PTB domain, and Leucine zipper motif) antibodies target a family of intracellular adaptor proteins, primarily APPL1 and APPL2. which play critical roles in signal transduction and membrane trafficking. Discovered in the early 2000s, APPL proteins interact with multiple signaling pathways, including those mediated by Akt, Rab5. and G-protein-coupled receptors, influencing cell survival, proliferation, and glucose metabolism. APPL1. the better-characterized isoform, links insulin signaling to adiponectin receptors, impacting metabolic homeostasis. Its dysfunction is implicated in diabetes, obesity, and cancer. APPL2 shares structural similarity but exhibits distinct functions, such as regulating endosomal trafficking and neuronal development. Antibodies against APPL proteins are essential tools for studying their localization, expression levels, and interactions in various tissues. They enable detection via Western blotting, immunofluorescence, and immunohistochemistry, aiding research into metabolic disorders, neurodegenerative diseases, and oncogenesis. Recent studies also explore APPL's role in autophagy and immune responses, expanding its relevance in biomedical research. Commercial APPL antibodies are typically validated for specificity using knockout cell lines or siRNA knockdowns, ensuring reliability in experimental models.

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