| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MDH s; mdh1; MDHA; MDHs; MOR2;;MDH1 |
| WB Predicted band size | 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MDH1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于MDH1抗体的3篇示例文献(内容为模拟生成，供参考)：
1. **《Development and Validation of a Monoclonal Antibody Targeting MDH1 for Metabolic Studies》**
- 作者：Li Y, Chen X
- 摘要：本研究成功开发了一种高特异性小鼠源单克隆MDH1抗体，验证了其在Western blot、免疫组化中的适用性，并证实其在细胞能量代谢研究中的作用，为代谢疾病模型提供了可靠工具。
2. **《MDH1 Expression in Colorectal Cancer: Correlation with Glycolytic Activity and Prognosis》**
- 作者：Wang H et al.
- 摘要：通过MDH1抗体检测结直肠癌组织中的蛋白表达水平，发现MDH1高表达与糖酵解增强及患者不良预后显著相关，提示其可能作为癌症代谢重编程的生物标志物。
3. **《Antibody-based Detection of MDH1 Oxidation in Neurodegenerative Disorders》**
- 作者：Garcia R, Kim S
- 摘要：利用MDH1抗体检测阿尔茨海默病模型中的氧化修饰MDH1蛋白，发现其异常积累与线粒体功能障碍相关，为神经退行性病变机制研究提供了新方向。
注：以上文献为示例，实际引用需检索PubMed、Web of Science等数据库获取真实文献。
The MDH1 antibody targets malate dehydrogenase 1 (MDH1), a key enzyme in the malate-aspartate shuttle and tricarboxylic acid (TCA) cycle. MDH1. the cytoplasmic isoform, catalyzes the reversible conversion of malate to oxaloacetate, linking glycolysis and mitochondrial energy production. It plays a vital role in cellular metabolism, redox balance, and biosynthetic pathways. MDH1 antibodies are widely used in research to study protein expression, localization, and function in various tissues and disease contexts.
These antibodies are essential tools for applications like Western blotting, immunohistochemistry, and immunofluorescence. Dysregulation of MDH1 has been implicated in cancers (e.g., colorectal, liver) due to metabolic reprogramming in tumor cells. Studies also explore its role in neurodegenerative disorders like Alzheimer’s disease, where altered mitochondrial function and oxidative stress are observed.
MDH1 antibodies aid in identifying its interaction partners and post-translational modifications, offering insights into regulatory mechanisms. Commercial MDH1 antibodies are typically validated for specificity using knockout cell lines or siRNA silencing. As a housekeeping gene product, MDH1 is often used as a loading control in experiments, emphasizing the need for high-quality antibodies to ensure experimental accuracy. Research continues to uncover its therapeutic potential in metabolic and age-related diseases.
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