| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CACH; CLE; EIF 2B; EIF2B; EIF2B5; EIF2BE; EIF2Bepsilon;;eIF2B5 |
| WB Predicted band size | 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human eIF2B5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于eIF2Bε抗体的3篇参考文献及其摘要内容的简要概括：
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1. **文献名称**：*Mutations in the EIF2Bepsilon subunit are associated with childhood ataxia with central hypomyelination (CACH)*
**作者**：van der Knaap, M.S., et al. (2002)
**摘要**：该研究首次报道了eIF2Bepsilon基因突变与脑白质营养不良(VWM)的关联，并开发了特异性eIF2Bepsilon抗体用于患者细胞分析，发现突变导致蛋白质功能异常，影响eIF2B复合物的活性。
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2. **文献名称**：*Decreased eIF2Bepsilon activity in vanishing white matter disease*
**作者**：Fogli, A., et al. (2003)
**摘要**：通过eIF2Bepsilon抗体检测患者细胞中的蛋白表达水平，研究发现VWM患者的eIF2B复合物活性显著降低，揭示了基因型与表型之间的关联，抗体被用于Western blot和免疫沉淀实验。
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3. **文献名称**：*eIF2Bepsilon subcellular localization and interaction in stress granules*
**作者**：Richardson, J.P., et al. (2005)
**摘要**：利用eIF2Bepsilon抗体进行免疫荧光染色，发现该蛋白在细胞应激条件下参与应激颗粒的形成，并与其他翻译调控因子共定位，提示其在蛋白质合成应激响应中的作用。
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4. **文献名称**：*Functional analysis of eIF2B complex mutations using isoform-specific antibodies*
**作者**：Wong, L.J., et al. (2018)
**摘要**：通过亚基特异性抗体(包括eIF2Bepsilon)分析突变对eIF2B复合物组装的影响，发现特定突变导致复合物稳定性下降，为开发针对eIF2B相关疾病的诊断工具提供了依据。
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这些文献均涉及eIF2Bepsilon抗体的实验应用，涵盖疾病机制、蛋白功能分析及诊断研究。
The eukaryotic initiation factor 2B epsilon (eIF2Bε) antibody is a critical tool for studying the regulation of protein synthesis, particularly in the context of cellular stress and neurological disorders. eIF2B, a five-subunit guanine nucleotide exchange factor (GEF), activates eIF2 by catalyzing the exchange of GDP for GTP during the initiation phase of mRNA translation. The epsilon subunit (eIF2Bε) is the largest and most regulatory component, directly interacting with eIF2 and serving as a primary target for phosphorylation under stress conditions, such as the integrated stress response (ISR). Dysregulation of eIF2B activity is linked to severe pathologies, including vanishing white matter (VWM) disease, a fatal leukoencephalopathy caused by mutations in the EIF2B1-5 genes, particularly EIF2B5 encoding the epsilon subunit.
eIF2Bε-specific antibodies are widely used in research to investigate eIF2B complex assembly, activity modulation, and its role in translational control. They enable detection of eIF2Bε expression levels, post-translational modifications (e.g., phosphorylation), and interactions with other cellular components via techniques like Western blotting, immunoprecipitation, and immunofluorescence. These antibodies are also vital for studying disease mechanisms in VWM models and exploring therapeutic interventions targeting the ISR pathway. Commercially available eIF2Bε antibodies are typically validated in human, mouse, and rat samples, with applications spanning basic molecular biology, neuropathology, and drug discovery research.
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