首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肌苷5-磷酸脱氢酶2 IMPDH2酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IMPDH2含量。IMPDH2 是重要靶点。它在细胞增殖、免疫调节等生理过程中发挥关键作用。研究中发现抑制 IMPDH2 能影响核苷酸合成，进而干扰肿瘤细胞生长和病毒复制，在肿瘤治疗和抗病毒治疗领域是研究热点，为相关疾病治疗提供新思路。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，支持科研人员开展嘌呤代谢相关机制研究、抗肿瘤药物筛选或免疫调节研究等实验。兼容多种生物样本类型，尤其适用于体外培养细胞模型的代谢状态评估或疾病动物模型中 IMPDH2 的动态监测，为探索靶点在疾病发生发展中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IMDH2_HUMAN ELISA Kit; IMP (inosine monophosphate) dehydrogenase 2 ELISA Kit; IMP dehydrogenase 2 ELISA Kit; IMP oxireductase 2 ELISA Kit; IMPD 2 ELISA Kit; IMPD2 ELISA Kit; IMPDH 2 ELISA Kit; IMPDH II ELISA Kit; IMPDH-II ELISA Kit; Impdh2 ELISA Kit; Inosine 5' monophosphate dehydrogenase 2 ELISA Kit; Inosine 5' phosphate dehydrogenase 2 ELISA Kit; Inosine monophosphate dehydrogenase type II ELISA Kit; Inosine-5''-monophosphate dehydrogenase 2 ELISA Kit
缩写：IMPDH2
Uniprot No.：P12268
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IMPDH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 88-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-102 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-91 | |||||
回收率：
| The recovery of human IMPDH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-102 | ||||
| EDTA plasma (n=4) | 88 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.6322.5102.5712.4487501.4761.5191.4981.3753750.8670.8200.8440.721187.50.4730.4650.4690.346940.3050.3110.3080.185470.2580.2460.2520.12923.50.1890.1850.1870.06400.1240.1220.123 | ||||||
货期：3-5 working days
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