| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ALOX5; 5-LO; 5-LOX; 5LPG; LOG5; MGC163204; LOX5; 5-lipoxygenase ; 5 Lipoxygenase; 5 LOX; ALOX 5;;5 Lipoxygenase |
| WB Predicted band size | 78 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human 5 Lipoxygenase |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于5-Lipoxygenase(5-LOX)抗体的3篇文献示例(文献信息为模拟虚构，仅供参考)：
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1. **文献名称**: *"Role of 5-Lipoxygenase in Neuroinflammatory Pathways: Antibody-Based Localization in Alzheimer's Disease Models"*
**作者**: Smith J, et al.
**摘要**: 该研究利用特异性5-LOX抗体，通过免疫组化技术揭示了5-LOX酶在阿尔茨海默病模型小鼠脑组织中的高表达，并证实其与神经炎症小胶质细胞活化的关联。
2. **文献名称**: *"Development of a Monoclonal Antibody Against 5-LOX for Targeted Inhibition in Colorectal Cancer"*
**作者**: Lee H, et al.
**摘要**: 研究团队开发了一种高亲和力的单克隆抗体，可特异性结合5-LOX酶活性位点，体外实验表明其能有效抑制结肠癌细胞中促炎介质白三烯的生成。
3. **文献名称**: *"5-Lipoxygenase Isoform-Specific Antibodies Reveal Differential Expression in Atherosclerotic Plaques"*
**作者**: Garcia R, et al.
**摘要**: 通过亚型特异性抗体分析，发现5-LOX不同剪接变体(如5-LOX、5-LOXΔ13)在动脉粥样硬化斑块中的差异分布，提示其在血管炎症中的异构体功能特异性。
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**备注**：上述文献为示例，实际引用需检索PubMed、Web of Science等平台获取真实数据，并注意选择近5年高被引研究或权威期刊(如*Journal of Immunology*、*PNAS*等)。
The 5-lipoxygenase (5-LO) antibody is a key tool for studying the enzyme 5-lipoxygenase, which plays a central role in the biosynthesis of leukotrienes (LTs), lipid mediators involved in inflammatory and immune responses. 5-LO catalyzes the conversion of arachidonic acid to leukotriene A4 (LTA4), a precursor for pro-inflammatory leukotrienes like LTB4 and cysteinyl-LTs (LTC4. LTD4. LTE4). These molecules are implicated in asthma, allergic reactions, cardiovascular diseases, and cancer progression. Antibodies targeting 5-LO are widely used in research to detect its expression, localization, and regulation in cells and tissues, aiding in understanding its pathophysiological roles.
5-LO antibodies are typically developed against specific epitopes of the enzyme, often validated for applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Their specificity allows researchers to study 5-LO expression patterns in immune cells (e.g., neutrophils, macrophages), epithelial cells, and cancer cells. Dysregulation of 5-LO activity is linked to chronic inflammatory diseases, atherosclerosis, and certain cancers, making these antibodies valuable for exploring therapeutic targets. Additionally, they help assess the efficacy of 5-LO inhibitors in preclinical models. Cross-reactivity with 5-LO orthologs in different species (e.g., human, mouse, rat) is often characterized to support translational studies. Proper validation using knockout controls or siRNA-mediated silencing ensures antibody reliability. Overall, 5-LO antibodies are indispensable for dissecting leukotriene-driven pathways and developing anti-inflammatory or anti-cancer strategies.
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