| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hSMURF2; SMUF2_HUMAN; Smurf2;;SMURF2 |
| WB Predicted band size | 86 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human SMURF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于SMURF2抗体的3篇参考文献示例(注：文献为虚构示例，仅供参考格式)：
---
1. **文献名称**：SMURF2 regulates TGF-β signaling and metastasis in triple-negative breast cancer
**作者**：Wang L, et al.
**摘要**：本研究探讨了SMURF2通过泛素化降解Smad7调控TGF-β信号通路在乳腺癌转移中的作用。通过Western blot(使用Santa Cruz的SMURF2抗体，sc-100616)和免疫共沉淀技术，发现SMURF2高表达与患者预后不良相关，靶向抑制SMURF2可减少肿瘤侵袭。
---
2. **文献名称**：SMURF2-mediated ubiquitination of PTEN promotes liver fibrosis
**作者**：Zhang Y, et al.
**摘要**：文章揭示了SMURF2在肝星状细胞活化中通过泛素化PTEN加剧纤维化的机制。采用免疫组化(兔源SMURF2抗体，Abcam ab12345)检测临床样本，发现SMURF2与纤维化程度正相关，体外实验证实其调控细胞增殖及ECM沉积。
---
3. **文献名称**：SMURF2 modulates autophagy in non-small cell lung cancer via Beclin1 regulation
**作者**：Chen H, et al.
**摘要**：该研究证明SMURF2通过泛素化修饰Beclin1抑制自噬，促进肺癌化疗耐药。实验使用小鼠单克隆SMURF2抗体(Cell Signaling #6783)进行免疫荧光和蛋白互作分析，提示靶向SMURF2可增强顺铂敏感性。
---
(注：以上文献信息为示例，实际引用请查询PubMed等数据库获取真实研究。)
The SMURF2 (SMAD-specific E3 ubiquitin protein ligase 2) antibody is a crucial tool for studying the role of SMURF2. a HECT-domain E3 ubiquitin ligase involved in regulating cellular processes like protein degradation, signal transduction, and cell homeostasis. SMURF2 primarily targets substrates for proteasomal degradation via ubiquitination, modulating pathways such as TGF-β/BMP signaling, DNA damage response, and planar cell polarity. It interacts with SMAD proteins (e.g., SMAD1/5/7), receptor-regulated effectors of TGF-β/BMP signaling, and non-SMAD targets like PAR4 and RNF11. influencing cell proliferation, differentiation, and apoptosis. Dysregulation of SMURF2 is linked to cancer, fibrosis, and developmental disorders.
SMURF2 antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect protein expression, localization, and interactions. These antibodies help researchers explore SMURF2's regulatory mechanisms, including its autoinhibition via N-terminal domains, substrate recognition, and post-translational modifications. Species reactivity typically covers human, mouse, and rat. Validated antibodies are critical for distinguishing SMURF2 isoforms or phosphorylation states, which affect its function. Commercial antibodies often include validation data (e.g., knockout cell line controls) to ensure specificity. Understanding SMURF2's dual roles—as both a tumor suppressor and promoter in context-dependent scenarios—highlights the antibody's importance in dissecting its complex biological and pathological contributions.
×
