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Human U3 small nucleolar ribonucleoprotein protein IMP3(IMP3) ELISA kit

  • 中文名： 人U3小核糖核蛋白IMP3(IMP3)ELISA kit
货号: IPD-EL011692HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人U3小核糖核蛋白IMP3(IMP3)酶联免疫试剂盒, 双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的IMP3含量。IMP3 是近年来受关注的靶点。它与肿瘤发生发展密切相关，在多种肿瘤组织中高表达。研究显示其可调控细胞增殖、凋亡与转移等过程，有望成为肿瘤诊断标志物和治疗靶点，为癌症精准治疗提供新思路。试剂盒检测范围为39 pg/mL-2500 pg/mL，本试剂盒通过高特异性抗体对实现目标蛋白的精准捕获与信号放大，可满足科研场景下对IMP3蛋白表达水平的定量分析需求，例如肿瘤生物学研究中的分子机制探索、细胞功能实验中的蛋白动态监测或疾病模型构建中的生物标志物验证等，为体外样本中IMP3的定量检测提供标准化工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    BRMS2 ELISA Kit; C15orf12 ELISA Kit; Chromosome 15 open reading frame 12 ELISA Kit; DKFZp586L0118 ELISA Kit; FLJ10968 ELISA Kit; imp3 ELISA Kit; IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast) ELISA Kit; IMP3_HUMAN ELISA Kit; Mitochondrial ribosomal protein S4 ELISA Kit; MRPS4 ELISA Kit; OTTHUMP00000183524 ELISA Kit; U3 small nucleolar ribonucleoprotein protein imp3 ELISA Kit; U3 snoRNP protein 3 homolog ELISA Kit; U3 snoRNP protein imp3 ELISA Kit
  • 缩写：
    IMP3
  • Uniprot No.：
    Q9NV31
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    39 pg/mL-2500 pg/mL
  • 灵敏度：
    9.75 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%



    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%



    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IMP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)

    1:1Average %95

    Range %89-99

    1:2Average %97

    Range %91-103

    1:4Average %92

    Range %87-98

    1:8Average %89

    Range %85-93

  • 回收率：
    The recovery of human IMP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange

    Serum (n=5)10298-105

    EDTA plasma (n=4)8680-91

















    标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.









































































































    pg/mlOD1OD2AverageCorrected

    25002.4452.5552.5002.344

    12501.7911.8611.8261.670

    6251.2321.2871.2601.104

    3120.8660.8770.8720.716

    1560.5800.5930.5870.431

    780.4370.4240.4310.275

    390.2830.2890.2860.130

    00.1550.1560.156










  • 货期：
    3-5 working days

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