| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PFK-B; PFK-L; ATP-PFK |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PFKL |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于HMGA1抗体的参考文献及其摘要概括：
1. **文献名称**：*HMGA1 is a novel downstream nuclear target of the ErbB2 signaling pathway*
**作者**：Chiappetta, G. et al.
**摘要**：该研究通过免疫沉淀(IP)和Western blot技术，利用HMGA1特异性抗体揭示了ErbB2信号通路如何调控HMGA1的磷酸化，促进乳腺癌细胞侵袭和转移的分子机制。
2. **文献名称**：*HMGA1 antibodies in the diagnosis of thyroid neoplasms*
**作者**：Tallini, G. et al.
**摘要**：研究验证了HMGA1抗体在甲状腺肿瘤组织中的免疫组化(IHC)应用，证明其高表达与甲状腺癌恶性程度相关，可作为鉴别良恶性肿瘤的生物标志物。
3. **文献名称**：*The role of HMGA1 in colorectal cancer stemness and drug resistance*
**作者**：Resar, L. et al.
**摘要**：通过流式细胞术和免疫荧光，利用HMGA1抗体发现其在结直肠癌干细胞中高表达，并与化疗耐药性相关，提示HMGA1为潜在治疗靶点。
4. **文献名称**：*HMGA1 chromatin regulators drive oncogenic reprogramming in hematologic malignancies*
**作者**：Fusco, A. et al.
**摘要**：采用ChIP-seq和HMGA1抗体分析白血病细胞，阐明HMGA1通过重塑染色质结构激活致癌基因，为血液肿瘤的靶向治疗提供依据。
以上研究均基于HMGA1抗体在不同技术场景(如IHC、Western blot、ChIP)中的应用，聚焦其在肿瘤发生、诊断及治疗中的机制与价值。
HMGA1 (High Mobility Group AT-Hook 1) is a non-histone chromatin protein involved in regulating DNA architecture and gene expression. It binds to AT-rich regions of DNA, modulating transcriptional activity by altering chromatin structure or facilitating the assembly of multiprotein complexes. HMGA1 is highly expressed during embryogenesis but is typically low or absent in most adult tissues. Dysregulation of HMGA1. particularly overexpression, is strongly associated with cancer progression, metastasis, and therapy resistance, making it a biomarker of interest in malignancies like breast, colon, and pancreatic cancers.
HMGA1 antibodies are essential tools for detecting and quantifying HMGA1 protein levels in research and diagnostics. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence to study HMGA1's role in tumorigenesis, epigenetic regulation, and cellular differentiation. These antibodies can differentiate between HMGA1 isoforms (HMGA1a and HMGA1b) or detect pan-HMGA1. depending on their design. Validation of specificity is critical, as cross-reactivity with related proteins (e.g., HMGA2) may occur. Commercially available HMGA1 antibodies are typically raised in rabbits or mice, with monoclonal and polyclonal variants offering distinct advantages in sensitivity and application flexibility. Recent studies also explore HMGA1's involvement in metabolic disorders and inflammatory diseases, expanding its therapeutic and diagnostic relevance.
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