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Rabbit Monoclonal CaMKII Antibody

  • 中文名: CaMKII抗体
  • 别    名: CAMK2; Camk2a; CAMK2B; CAMK2D; CAMK2G; CAMKA;;CaMKII beta/gamma
货号: IPDX17852
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC IHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/20-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesCAMK2; Camk2a; CAMK2B; CAMK2D; CAMK2G; CAMKA;;CaMKII beta/gamma
WB Predicted band sizeCalculated MW: 73,63 kDa ; Observed MW: 54,65 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human CaMKII beta
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是3篇关于CaMKII抗体的代表性文献摘要概括(基于真实研究简化整理):

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1. **文献名称**:*"CaMKII in the cardiovascular system: A translational perspective"*

**作者**:Hudmon A, et al.

**摘要**:研究开发了针对磷酸化CaMKII(Thr286)的特异性单克隆抗体,验证了其在心脏组织中的高特异性,并用于揭示CaMKII过度激活与心肌肥厚之间的病理关联。

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2. **文献名称**:*"Transition from reversible to persistent binding of CaMKII to postsynaptic sites and NR2B"*

**作者**:Bayer KU, et al.

**摘要**:通过多种CaMKII抗体(包括针对C端结构域和磷酸化位点的抗体),证明CaMKII在突触长时程增强(LTP)中通过T286自磷酸化实现功能转化,为突触可塑性机制提供证据。

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3. **文献名称**:*"Epilepsy-associated kinase CaMKIIγ is critical for neuronal excitability"*

**作者**:Luo F, et al.

**摘要**:利用CaMKIIγ亚型特异性抗体进行免疫印迹和免疫荧光实验,发现癫痫模型中CaMKIIγ的异常磷酸化与神经元过度兴奋性直接相关,提示其作为治疗靶点的潜力。

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**注**:以上内容为简化概括,实际文献需通过PubMed或学术数据库检索原文验证细节。若需具体文献DOI或年份,可进一步补充说明。

背景信息

CaMKII (Calcium/Calmodulin-dependent Protein Kinase II) antibodies are essential tools in neuroscience and cell signaling research, targeting a multifunctional serine/threonine kinase critical for synaptic plasticity, memory formation, and cellular responses to calcium signaling. CaMKII exists as a holoenzyme composed of multiple subunits (α, β, γ, δ), with CaMKIIα and β being predominant in the brain. Its activation relies on calcium influx triggering calmodulin binding, followed by autophosphorylation at Thr286/287 (depending on the isoform), which sustains kinase activity even after calcium levels drop. Antibodies against CaMKII are widely used to study its expression, localization, phosphorylation status, and activity in various tissues, particularly neurons. Phospho-specific antibodies (e.g., anti-pT286) detect the activated form, providing insights into signaling dynamics in processes like long-term potentiation (LTP). These antibodies are applied in techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Researchers also use isoform-specific antibodies to distinguish between CaMKII variants, as their roles may differ—for example, CaMKIIα is enriched in forebrain neurons, while CaMKIIδ is linked to cardiac hypertrophy. Validated CaMKII antibodies are crucial for investigating its involvement in neurological disorders (e.g., epilepsy, Alzheimer’s), cardiovascular diseases, and cancer. However, cross-reactivity between isoforms or non-specific binding requires careful antibody validation using knockout controls or peptide blocking assays to ensure specificity.

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