| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Insulin receptor substrate 2; IRS 2; IRS-2;;Insulin receptor substrate 2 |
| WB Predicted band size | Calculated MW: 137 kDa ; Observed MW: 185 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Insulin receptor substrate 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于IRS2抗体的3篇参考文献，格式为文献名称、作者及摘要概括：
1. **"IRS2-mediated β-cell survival in response to high-fat diet"**
*作者：Withers, D.J. 等*
摘要：研究通过IRS2敲除小鼠模型，利用IRS2抗体进行Western blot分析，揭示高脂饮食下IRS2对胰岛β细胞存活的关键作用，提示其与2型糖尿病发展的关联。
2. **"Role of IRS2 in tumor angiogenesis and colorectal cancer progression"**
*作者：Nagayama, M. 等*
摘要：通过免疫组化(使用IRS2抗体)分析结直肠癌组织，发现IRS2过表达与肿瘤血管生成相关，表明靶向IRS2可能抑制癌症进展。
3. **"IRS2 regulates mitochondrial dynamics in neurons"**
*作者：Kim, B. 等*
摘要：研究采用IRS2抗体进行免疫荧光染色，发现IRS2通过调控线粒体分裂蛋白Drp1影响神经元能量代谢，可能参与神经退行性疾病机制。
注：以上信息为示例，实际文献需通过PubMed或Google Scholar检索确认。建议使用关键词“IRS2 antibody”或“IRS2 immunohistochemistry”查找具体实验应用文献。
The insulin receptor substrate 2 (IRS2) antibody is a crucial tool in studying insulin and insulin-like growth factor (IGF) signaling pathways. IRS2. a cytoplasmic adaptor protein, acts as a mediator in transmitting signals from activated insulin/IGF-1 receptors to downstream effectors involved in metabolism, growth, and survival. It contains multiple tyrosine phosphorylation sites that recruit signaling molecules such as PI3K and Grb2. facilitating pathways regulating glucose homeostasis, cell proliferation, and apoptosis. Dysregulation of IRS2 is linked to metabolic disorders like type 2 diabetes, obesity, and certain cancers, making it a focal point in metabolic and oncological research.
IRS2 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to detect IRS2 expression, post-translational modifications (e.g., phosphorylation), and subcellular localization. These antibodies help elucidate IRS2's role in insulin resistance, pancreatic β-cell function, and tumor progression. Research has also highlighted IRS2's involvement in neuronal survival and cognitive function, connecting it to neurodegenerative diseases. Species-specific IRS2 antibodies (human, mouse, rat) enable cross-disciplinary studies, while validation via knockout/knockdown controls ensures specificity. Overall, IRS2 antibodies are indispensable for unraveling the molecular mechanisms of metabolic regulation and disease pathogenesis.
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