| WB | 咨询技术 | Mouse,Rat |
| IF | 1/20-1/50 | Mouse,Rat |
| IHC | 咨询技术 | Mouse,Rat |
| ICC | 技术咨询 | Mouse,Rat |
| FCM | 咨询技术 | Mouse,Rat |
| Elisa | 咨询技术 | Mouse,Rat |
| Aliases | UCP1; UCP3;;UCP1/3 |
| WB Predicted band size | Calculated MW: 33,34 kDa ; Observed MW: 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse,Rat |
| Immunogen | A synthesized peptide derived from mouse UCP1/3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于UCP1和UCP3抗体的3-4条参考文献示例(内容为虚构，仅供参考格式)：
1. **文献名称**: "Specific Detection of UCP1 in Brown Adipose Tissue Using a Novel Polyclonal Antibody"
**作者**: Ricquier D., Bouillaud F.
**摘要**: 该研究开发了一种针对UCP1蛋白C端结构域的多克隆抗体，通过免疫组化和Western blot验证其在棕色脂肪组织中的特异性，排除了与UCP2/UCP3的交叉反应，为产热研究提供可靠工具。
2. **文献名称**: "Characterization of UCP3 Expression in Skeletal Muscle via Monoclonal Antibody-Based Assays"
**作者**: Boss O., et al.
**摘要**: 作者利用单克隆抗体分析UCP3在小鼠和人类骨骼肌中的表达，证实其在能量代谢中的潜在作用，并验证抗体在免疫沉淀实验中的高特异性。
3. **文献名称**: "Comparative Analysis of UCP1 and UCP3 Antibodies Across Species: Implications for Metabolic Disease Models"
**作者**: Gong D.W., et al.
**摘要**: 研究评估了UCP1/UCP3抗体在小鼠、大鼠和人类样本中的交叉反应性，发现部分抗体存在物种特异性差异，为代谢疾病模型选择合适抗体提供依据。
4. **文献名称**: "Role of UCP1 in Adaptive Thermogenesis: Antibody Validation in Obesity Studies"
**作者**: Bachman E.S., et al.
**摘要**: 通过Western blot和免疫荧光验证UCP1抗体在肥胖模型小鼠棕色脂肪组织中的敏感性，揭示UCP1表达水平与产热能力的相关性。
(注：以上文献为示例模板，实际引用需检索真实数据库如PubMed，并核实作者及内容。)
UCP1 (Uncoupling Protein 1) and UCP3 (Uncoupling Protein 3) are mitochondrial membrane proteins involved in thermogenesis and energy metabolism. UCP1. primarily expressed in brown adipose tissue (BAT), uncouples oxidative phosphorylation from ATP production, dissipating energy as heat—a process critical for non-shivering thermogenesis. UCP3. found in skeletal muscle and BAT, shares structural homology with UCP1 but has distinct roles, including fatty acid metabolism, reactive oxygen species regulation, and potential thermogenic functions under specific conditions. Antibodies targeting UCP1 and UCP3 are essential tools for studying their expression, localization, and regulatory mechanisms in metabolic tissues.
These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate their roles in obesity, diabetes, and metabolic disorders. UCP1 antibodies help assess BAT activity and adaptive thermogenesis, while UCP3 antibodies explore its involvement in muscle energy expenditure and mitochondrial efficiency. Species specificity (e.g., human, mouse, rat) is a key consideration, as cross-reactivity varies. Researchers also utilize these antibodies to study UCP1/3 modulation by hormones, cold exposure, or pharmacological agents, providing insights into therapeutic strategies for metabolic diseases. Validated antibodies ensure accurate detection, distinguishing between isoforms to avoid misinterpretation in studies on tissue-specific energy homeostasis.
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