| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hREV7; MAD2B; Mad2l2 Mitotic Arrest Deficient 2 L2; POLZ2; REV 7; REV7; REV7 homolog;;MAD2L2 |
| WB Predicted band size | 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MAD2L2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与Mad2L2(MAD2B)抗体相关的参考文献摘要：
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1. **文献名称**: "MAD2B is an inhibitor of the anaphase-promoting complex"
**作者**: Chen J, et al.
**摘要**: 该研究通过免疫沉淀和Western blot验证了MAD2B抗体的特异性，发现MAD2B通过抑制APC/C复合物参与细胞周期调控，并在DNA损伤修复中发挥作用。
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2. **文献名称**: "Mad2B upregulation promotes tumor progression in hepatocellular carcinoma"
**作者**: Li Y, et al.
**摘要**: 利用抗MAD2L2抗体进行免疫组化(IHC)分析，发现肝癌组织中MAD2B表达升高与患者预后不良相关，提示其可作为潜在治疗靶点。
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3. **文献名称**: "Structural basis of Mad2L2 recognition by the error-prone DNA polymerase ζ"
**作者**: Xie J, et al.
**摘要**: 通过抗体介导的免疫荧光(IF)和共定位实验，揭示了MAD2L2与DNA聚合酶ζ的相互作用机制，阐明其在跨损伤DNA合成中的调控作用。
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4. **文献名称**: "A novel role of Mad2L2 in ovarian cancer chemoresistance"
**作者**: Wang H, et al.
**摘要**: 采用MAD2L2抗体进行体外功能实验，证明其通过调控同源重组修复通路导致卵巢癌细胞对化疗药物产生耐药性。
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这些文献均涉及MAD2L2抗体的实验应用(如WB、IHC、IF等)，并聚焦于其在细胞周期、癌症及DNA修复中的功能机制。如需具体文献来源(期刊、年份)，可进一步补充关键词检索。
Mad2L2 (Mad2-like protein 2), also known as REV7. is a multifunctional protein involved in diverse cellular processes, including DNA damage repair, cell cycle regulation, and chromosome segregation. It acts as a critical component of the translesion synthesis (TLS) pathway, where it partners with DNA polymerase ζ (Polζ) to bypass DNA lesions during replication, thereby maintaining genomic stability. Additionally, Mad2L2 interacts with the shieldin complex in DNA double-strand break repair, influencing the choice between non-homologous end joining (NHEJ) and homologous recombination (HR).
Antibodies targeting Mad2L2 are essential tools for studying its roles in these pathways. They are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect Mad2L2 expression, localization, and interactions in various biological contexts. Dysregulation of Mad2L2 has been linked to cancer progression, chemoresistance, and genomic instability, making its antibodies valuable in cancer research and therapeutic development. These antibodies also help explore Mad2L2’s emerging functions in meiosis, neurodegeneration, and immune regulation. By enabling precise detection and functional analysis, Mad2L2 antibodies contribute to understanding cellular stress responses and developing strategies to target replication and repair vulnerabilities in diseases.
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