Mouse Interleukin 5,IL-5 ELISA KIT

产品详情

中文名： 小鼠白介素-5(IL-5)ELISA Kit

货号: IPD-E04637m

Price： ￥2500

48T 96T

数量：

大包装询价

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：
小鼠白介素-5(IL-5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL5含量。IL5 是重要的治疗靶点。它主要由 Th2 细胞产生，能促进嗜酸性粒细胞生长、活化及存活。在哮喘、嗜酸性粒细胞增多症等疾病中，IL5 水平常异常升高。针对 IL5 开发的药物，通过阻断其活性来减少嗜酸性粒细胞，改善相关疾病症状。试剂盒检测范围为15.6 pg/ml- 1000 pg/ml，灵敏度为3.9 pg/ml。适用于免疫调控机制研究、炎症性疾病模型评估以及药物干预实验中细胞因子通路的分析；广泛用于基础免疫学、病理机制探索及生物制剂开发等科研领域，帮助解析IL - 5在特定生理或病理状态下的生物学功能。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Il5 ELISA Kit; Il-5Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
缩写：
IL5
Uniprot No.：
P04401
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
15.6 pg/ml - 1000 pg/ml
灵敏度：
3.9 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	82
	Range %	80-92
1:2	Average %	89
	Range %	85-100
1:4	Average %	92
	Range %	90-105
1:8	Average %	90
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
1000	2.346	2.434	2.390	2.244
500	1.725	1.767	1.746	1.600
250	1.285	1.328	1.307	1.161
125	0.910	0.929	0.920	0.774
62.5	0.553	0.521	0.537	0.391
31.2	0.325	0.343	0.334	0.188
15.6	0.216	0.204	0.210	0.064
0	0.147	0.145	0.146